About: Comparison five primer sets from different genome region of COVID-19 for detection of virus infection by conventional RT-PCR

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Comparison five primer sets from different genome region of COVID-19 for detection of virus infection by conventional RT-PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparison five primer sets from different genome region of COVID-19 for detection of virus infection by conventional RT-PCR
Creator	Fazlalipour, Mehdi Aflatoonian, Behnaz Kalantar-Neyestanaki, Davood Mollaei, Hamid Aghaei Afshar, Abass
Source	Medline; PMC
abstract	BACKGROUND AND OBJECTIVES: The new beta-coronavirus, which caused Severe Acute Respiratory Coronavirus-2 Syndrome (SARS-CoV-2), a major respiratory outbreak in Wuhan, China in December 2019, is now prevalent in many countries around the world. Identifying PCR-based viruses is a well-known and relatively stable protocol. Unfortunately, the high mutation rates may lead to widespread changes in viral nucleic acid sequences, and so using specific primers for PCR can be recommended. In this study, we evaluated the power of a conventional RT-PCR to detect SARS-CoV-2 RNA among the five set primer sets. MATERIALS AND METHODS: The five genomic regions of the Coronavirus SARS-2 virus including Nucleocapsids (N), Envelope (E), RNA depended RNA Polymerase (RdRp), ORF1ab and Spike (S) were selected for primer designing. A conventional RT-PCR was performed to compare sensitivity, specificity and other analytical characteristics of primers designed against two Real Time PCR commercial kits. RESULTS: The result of the comparative analysis showed that the ORF1ab, N and RdRp primers had a sensitivity, specificity and positive predictive value higher than other primers. A significant difference in the analytical sensitivity between the studied primer sets in RT-PCR kits was observed. CONCLUSION: In this study, the ORF1ab, Nucleocapsid and RdRp regions have the best primers for identifying the SARS-CoV-2 RNA between different genes that have been suggested.
has issue date	2020-06-01(xsd:dateTime)
bibo:pmid	32685113
has license	cc-by
sha1sum (hex)	f1f39ac8b5872b9b02c34f726b089013f72d7318
resource representing a document's title	Comparison five primer sets from different genome region of COVID-19 for detection of virus infection by conventional RT-PCR
has PubMed Central identifier	PMC7340604
has PubMed identifier	32685113
schema:publication	Iran J Microbiol
resource representing a document's body	covid:f1f39ac8b5872b9b02c34f726b089013f72d7318#body_text
is schema:about of	named entity 'caused' named entity 'RdRp' named entity 'identifying' named entity 'Spike' named entity 'December' named entity 'primer' named entity 'Wuhan' named entity 'virus' named entity 'POWER' named entity 'METHODS' named entity 'BEST' named entity 'VIRUSES' named entity 'CHANGES' named entity 'COMMERCIAL' named entity 'NUCLEOCAPSIDS' named entity 'SET' named entity 'NUCLEOCAPSID' named entity 'WIDESPREAD' named entity 'CONVENTIONAL' named entity 'PCR' covid:arg/f1f39ac8b5872b9b02c34f726b089013f72d7318 named entity 'SARS-CoV-2' named entity 'primer' named entity 'suggested' named entity 'RdRp' named entity 'Conclusion' named entity 'outbreak' named entity 'PCR' named entity 'primers' named entity 'studied' named entity 'primers' named entity 'Coronavirus' named entity 'specificity' named entity 'Coronavirus' named entity 'Identifying' named entity 'SARS-CoV-2' named entity 'primers' named entity 'Nucleocapsids' named entity 'virus' named entity 'primer' named entity 'significant difference' named entity 'RNA' named entity 'China' named entity 'primers' named entity 'Nucleocapsids' named entity 'primer' named entity 'RT-PCR' named entity 'RT-PCR' named entity 'PCR' named entity 'SARS-CoV2' named entity 'genome' named entity 'Huanan seafood market' named entity 'coronary disease' named entity 'gene' named entity 'COVID-19' named entity 'COVID' named entity 'SARS' named entity 'gene' named entity 'cell culture' named entity 'virus' named entity 'COVID-19' named entity '2019-nCoV' named entity 'RNA' named entity 'coronavirus' named entity 'coronavirus infection' named entity 'nasopharyngeal swab' named entity 'ORF1' named entity 'viruses' named entity 'probability' named entity 'gene' named entity 'RdRp' named entity 'primers' named entity 'final target' named entity 'RT-PCR' named entity 'nucleocapsid' named entity 'sensitive, and specific' named entity 'nucleocapsid' named entity 'RNA' named entity 'virus'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software