About: Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion
Creator	Weiner, Leslie Sakaguchi, Alan Stohlman,', Stephen
Source	Elsevier; Medline; PMC
abstract	Abstract Mouse hepatitis virus, a coronavirus, was rescued from latently infected mouse neuroblastoma cells by polyethylene glycol-induced fusion to permissive cells. The isolated viruses grew to equal or higher titers than parental JHM virus at 39° but were restricted in replication at 32° and hence were cold-sensitive mutants. Neither isolate synthesized RNA at the nonpermissive temperature (32°), and temperature shift experiments indicated that the restrictive cold-sensitive step in replication occurred during an early viral function. The isolates were unable to complement each other at 32°. Although coinfection between each mutant and parental JHM virus often resulted in decreased yields of both infecting viruses, interference could not be convincingly demonstrated. Both mutants were more thermostable than parental JHM virus and could be distinguished from each other and from parental JHM virus by their thermal inactivation kinetics. The isolates were obtained from single cell clones and therefore presumed to be homogeneous; however, thermal inactivation of one (S-3JHM) indicated the possible existence of a second population. Subclones of virus prepared from each isolate were also found to be thermally stable and to retain the cold sensitive defect. No indication of a second population of S-3JHM virus was detected.
has issue date	1979-10-30(xsd:dateTime)
bibo:doi	10.1016/0042-6822(79)90567-1
bibo:pmid	228481
has license	els-covid
sha1sum (hex)	f21c95ba9743114144b44a49985e05a405920b29
schema:url	https://doi.org/10.1016/0042-6822%2879%2990567-1
resource representing a document's title	Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion
has PubMed Central identifier	PMC7130992
has PubMed identifier	228481
schema:publication	Virology
resource representing a document's body	covid:f21c95ba9743114144b44a49985e05a405920b29#body_text
is schema:about of	named entity 'CHARACTERIZATION' named entity 'SENSITIVE' named entity 'infecting' named entity 'Neither' named entity 'latently infected' named entity 'population' named entity 'kinetics' named entity 'defect' named entity 'mutants' named entity 'MUTANTS' named entity 'PARENTAL' named entity 'OBTAINED' named entity 'NO INDICATION OF' named entity 'SINGLE CELL' named entity 'SENSITIVE' named entity 'CORONAVIRUS' named entity 'VIRUSES' named entity 'RETAIN' named entity 'THERMAL' named entity 'TEMPERATURE' named entity 'SHIFT' named entity 'THEIR' named entity 'INFECTED' covid:arg/f21c95ba9743114144b44a49985e05a405920b29 named entity 'CELL FUSION' named entity 'MUTANTS' named entity 'COLD SENSITIVE' named entity 'RESTRICTIVE' named entity 'MUTANT' named entity 'COINFECTION' named entity 'CELLS' named entity 'OCCURRED' named entity 'CELLS' named entity 'VIRUS' named entity 'POLYETHYLENE' named entity 'MOUSE HEPATITIS VIRUS' named entity 'SECOND' named entity 'REPLICATION' named entity 'INACTIVATION KINETICS' named entity 'DECREASED' named entity 'INACTIVATION' named entity 'DETECTED' named entity 'ISOLATED' named entity 'PRESUMED' named entity 'ISOLATE' named entity 'EARLY' named entity 'HIGHER' named entity 'RNA' named entity 'STABLE' named entity 'MURINE HEPATITIS VIRUS' named entity '28S' named entity 'SYNTHESIZED' named entity 'DEMONSTRATED' named entity 'DEFECT' named entity 'FOUND' named entity 'COULD BE' named entity 'EQUAL' named entity 'PREPARED' named entity 'INDUCED' named entity 'BUT' named entity 'HOMOGENEOUS' named entity 'COMPLEMENT' named entity 'INFECTED' named entity 'CELL CLONES' named entity 'POSSIBLE' named entity 'VIRAL FUNCTION' named entity 'EXPERIMENTS' named entity 'INDICATED' named entity 'STEP' named entity 'NEITHER' named entity 'POPULATION' named entity 'FUSION' named entity 'NEUROBLASTOMA CELLS' named entity 'RESTRICTED' named entity 'MOUSE NEUROBLASTOMA' named entity 'INTERFERENCE' named entity 'COLD' named entity 'COLD' named entity 'coronavirus'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software