About: miR-1306 Mediates the Feedback Regulation of the TGF-β/SMAD Signaling Pathway in Granulosa Cells

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About: miR-1306 Mediates the Feedback Regulation of the TGF-β/SMAD Signaling Pathway in Granulosa Cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	miR-1306 Mediates the Feedback Regulation of the TGF-β/SMAD Signaling Pathway in Granulosa Cells
Creator	Yang, Liu Cao, Qiuyu Du, Xing Li, Qifa Liu, Lu Pan, Zengxiang
Source	PMC
abstract	Transforming growth factor-β receptor II (TGFBR2), the type II receptor of the TGF-β/SMA- and MAD-related protein (SMAD) signaling pathway, plays a crucial role in TGF-β signal transduction and is regulated by multiple factors. Nevertheless, the modulation of the non-coding RNA involved in the process of TGFBR2 expression in ovaries is not well studied. In our study, we isolated and characterized the 3′-untranslated region (UTR) of the porcine TGFBR2 gene and microRNA-1306 (miR-1306) was identified as the functional miRNA that targets TGFBR2 in porcine granulosa cells (GCs). Functional analysis showed that miR-1306 promotes apoptosis of GCs as well as attenuating the TGF-β/SMAD signaling pathway targeting and impairing TGFBR2 in GCs. Moreover, we identified the miR-1306 core promoter and found three potential SMAD4-binding elements (SBEs). Luciferase and chromatin immunoprecipitation (ChIP) assays revealed that the transcription factor SMAD4 directly binds to the miR-1306 core promoter and inhibits its transcriptional activity. Furthermore, the TGF-β/SMAD signaling pathway is modulated by SMAD4 positive feedback via inhibition of miR-1306 expression in GCs. Collectively, our findings provide evidence of an epigenetic mechanism that modulates as well as mediates the feedback regulation of the classical TGF-β/SMAD signaling pathway in GCs from porcine ovaries.
has issue date	2019-03-31(xsd:dateTime)
bibo:doi	10.3390/cells8040298
bibo:pmid	30935128
has license	cc-by
sha1sum (hex)	f29135f51da3a6598b511bad1514be9195bf1624
schema:url	https://doi.org/10.3390/cells8040298
resource representing a document's title	miR-1306 Mediates the Feedback Regulation of the TGF-β/SMAD Signaling Pathway in Granulosa Cells
has PubMed Central identifier	PMC6523565
has PubMed identifier	30935128
schema:publication	Cells
resource representing a document's body	covid:f29135f51da3a6598b511bad1514be9195bf1624#body_text
is schema:about of	named entity 'signaling pathway' named entity 'revealed' named entity 'protein' named entity 'TGF-β' named entity 'SMAD4' named entity 'apoptosis' named entity 'evidence' named entity 'transcriptional activity' named entity 'Nevertheless' named entity 'FUNCTIONAL' named entity 'ITS' named entity 'ISOLATED' named entity 'TYPE II RECEPTOR' named entity 'MEDIATES' named entity 'IDENTIFIED' named entity 'TGF' named entity 'FEEDBACK REGULATION' named entity 'MEDIATES' named entity 'POSITIVE FEEDBACK' named entity 'SMAD4' named entity 'DIRECTLY' named entity 'TARGETING' named entity 'OVARIES' named entity 'INHIBITION' named entity 'CORE PROMOTER' named entity 'CHROMATIN IMMUNOPRECIPITATION' named entity 'GCS' named entity 'RELATED' named entity 'REGULATED' named entity 'TGFBR2 GENE' named entity 'GRANULOSA CELLS' named entity 'STUDY' named entity 'SIGNALING PATHWAY' named entity 'EVIDENCE OF' named entity 'ELEMENTS' named entity 'EXPRESSION' named entity 'INVOLVED' named entity 'MIR-1306' named entity 'MODULATION' named entity 'MIR-1306' named entity 'SIGNALING PATHWAY' named entity '1306' named entity 'MAD' named entity 'PROCESS' named entity 'NOT WELL' named entity 'APOPTOSIS' named entity 'CLASSICAL' named entity 'LUCIFERASE' named entity 'EPIGENETIC MECHANISM' named entity 'TRANSCRIPTION FACTOR' named entity 'PROVIDE' named entity 'FOUND' named entity 'BINDING' named entity 'ROLE' named entity 'OUR' named entity 'IMPAIRING' named entity 'SIGNAL TRANSDUCTION' named entity 'UNTRANSLATED REGION' named entity 'FUNCTIONAL ANALYSIS' named entity 'PLAYS' named entity 'TRANSCRIPTIONAL ACTIVITY' named entity 'TRANSFORMING GROWTH FACTOR' named entity 'CHARACTERIZED' named entity 'POTENTIAL' named entity 'B2 RECEPTOR' named entity 'FINDINGS' named entity 'TGFBR2' named entity 'MICRORNA' named entity 'PROTEIN ' named entity 'TGF' named entity 'MODULATED' named entity 'GRANULOSA CELLS' named entity 'PORCINE' named entity 'NON-CODING RNA' named entity 'MULTIPLE' named entity 'TARGETS'

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