About: Generating Vesicular Stomatitis Virus Pseudotype Bearing the Severe Acute Respiratory Syndrome Coronavirus Spike Envelope Glycoprotein for Rapid and Safe Neutralization Test or Cell‐Entry Assay

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About: Generating Vesicular Stomatitis Virus Pseudotype Bearing the Severe Acute Respiratory Syndrome Coronavirus Spike Envelope Glycoprotein for Rapid and Safe Neutralization Test or Cell‐Entry Assay Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Generating Vesicular Stomatitis Virus Pseudotype Bearing the Severe Acute Respiratory Syndrome Coronavirus Spike Envelope Glycoprotein for Rapid and Safe Neutralization Test or Cell‐Entry Assay
Creator	Kong, Xiangang Hu, Sen Bu, Zhigao Chen, Hualan Ge, Jinying Liu, Yonggang Wang, Xijun Wen, Zhiyun
Source	Medline; PMC
abstract	abstract: We generated a recombinant vesicular stomatitis virus (VSV) pseudotype (VSV Δ GSG) by replacing the envelope G gene with the GFP gene and complementing with spike glycoprotein (S) of SARS‐CoV in trans. The neutralization and infection blocking tests showed that the VSV Δ GSG and SARS‐CoV reacted similarly to SARS‐CoV specific antiserum, suggesting the VSVΔ G*SG can be a safe replacement of the live SARS‐CoV for neutralization test and cell‐entry assay.
has issue date	2006-11-17(xsd:dateTime)
bibo:doi	10.1196/annals.1373.030
bibo:pmid	17135519
has license	no-cc
sha1sum (hex)	f329cdb26febc752b5fb19780e9a3a8d0d99c037
schema:url	https://doi.org/10.1196/annals.1373.030
resource representing a document's title	Generating Vesicular Stomatitis Virus Pseudotype Bearing the Severe Acute Respiratory Syndrome Coronavirus Spike Envelope Glycoprotein for Rapid and Safe Neutralization Test or Cell‐Entry Assay
has PubMed Central identifier	PMC7167956
has PubMed identifier	17135519
schema:publication	Ann N Y Acad Sci
resource representing a document's body	covid:f329cdb26febc752b5fb19780e9a3a8d0d99c037#body_text
is schema:about of	named entity 'spike' named entity 'SARS-CoV' named entity 'Rapid' named entity 'Vesicular Stomatitis Virus' named entity 'Assay' named entity 'REPLACING' named entity 'SARS-COV' named entity 'GFP' covid:arg/f329cdb26febc752b5fb19780e9a3a8d0d99c037 named entity 'blocking' named entity 'tests' named entity 'specific' named entity 'envelope' named entity 'SARS-CoV' named entity 'vesicular stomatitis virus' named entity 'Pseudotype' named entity 'VSV' named entity 'cytoplasmic' named entity 'amino acids' named entity 'VSV' named entity 'infectivity' named entity 'gene' named entity 'GFP' named entity '293T cells' named entity 'SARS' named entity 'glycoprotein' named entity 'Western blot' named entity 'SARS' named entity 'gene' named entity 'SARS' named entity 'assay' named entity 'vaccine' named entity 'supernatant' named entity 'SARS' named entity 'SARS' named entity 'antiviral drugs' named entity 'specific pathogen free' named entity 'SARS' named entity 'vaccine' named entity 'severe acute respiratory syndrome coronavirus' named entity 'assay' named entity 'Serum' named entity 'serum' named entity 'Vero E6' named entity 'VSV' named entity 'bio-safety level 3' named entity 'virus' named entity 'SARS' named entity 'fluorescence' named entity 'SARS' named entity 'VSV' named entity 'cytopathic effect' named entity 'VSV' named entity 'virus' named entity 'titer' named entity 'vesicular stomatitis virus' named entity 'fluorescence microscope' named entity 'antibodies' named entity 'neutralization test' named entity 'VSV' named entity 'Vero E6' named entity 'SARS' named entity 'VSV' named entity 'recombinant' named entity 'GFP' named entity 'membrane fusion' named entity 'GFP' named entity 'neutralization test' named entity 'VSV' named entity 'SARS' named entity 'Vero E6' named entity 'serum' named entity 'GFP' named entity 'VSV'

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