About: Metabolic engineering of Escherichia coli into a versatile glycosylation platform: production of bio-active quercetin glycosides

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About: Metabolic engineering of Escherichia coli into a versatile glycosylation platform: production of bio-active quercetin glycosides Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Metabolic engineering of Escherichia coli into a versatile glycosylation platform: production of bio-active quercetin glycosides
Creator	De Bruyn, Frederik De Mey, Marjan Maertens, Jo Van Brempt, Maarten Bellegem, Van
Source	Medline; PMC
abstract	BACKGROUND: Flavonoids are bio-active specialized plant metabolites which mainly occur as different glycosides. Due to the increasing market demand, various biotechnological approaches have been developed which use Escherichia coli as a microbial catalyst for the stereospecific glycosylation of flavonoids. Despite these efforts, most processes still display low production rates and titers, which render them unsuitable for large-scale applications. RESULTS: In this contribution, we expanded a previously developed in vivo glucosylation platform in E. coli W, into an efficient system for selective galactosylation and rhamnosylation. The rational of the novel metabolic engineering strategy constitutes of the introduction of an alternative sucrose metabolism in the form of a sucrose phosphorylase, which cleaves sucrose into fructose and glucose 1-phosphate as precursor for UDP-glucose. To preserve these intermediates for glycosylation purposes, metabolization reactions were knocked-out. Due to the pivotal role of UDP-glucose, overexpression of the interconverting enzymes galE and MUM4 ensured the formation of both UDP-galactose and UDP-rhamnose, respectively. By additionally supplying exogenously fed quercetin and overexpressing a flavonol galactosyltransferase (F3GT) or a rhamnosyltransferase (RhaGT), 0.94 g/L hyperoside (quercetin 3-O-galactoside) and 1.12 g/L quercitrin (quercetin 3-O-rhamnoside) could be produced, respectively. In addition, both strains showed activity towards other promising dietary flavonols like kaempferol, fisetin, morin and myricetin. CONCLUSIONS: Two E. coli W mutants were engineered that could effectively produce the bio-active flavonol glycosides hyperoside and quercitrin starting from the cheap substrates sucrose and quercetin. This novel fermentation-based glycosylation strategy will allow the economically viable production of various glycosides. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-015-0326-1) contains supplementary material, which is available to authorized users.
has issue date	2015-09-16(xsd:dateTime)
bibo:doi	10.1186/s12934-015-0326-1
bibo:pmid	26377568
has license	cc-by
sha1sum (hex)	f4cd52975e6aa33e8c947082eda9b261952b0f8f
schema:url	https://doi.org/10.1186/s12934-015-0326-1
resource representing a document's title	Metabolic engineering of Escherichia coli into a versatile glycosylation platform: production of bio-active quercetin glycosides
has PubMed Central identifier	PMC4573293
has PubMed identifier	26377568
schema:publication	Microb Cell Fact
resource representing a document's body	covid:f4cd52975e6aa33e8c947082eda9b261952b0f8f#body_text
is schema:about of	named entity 'developed' named entity 'PLATFORM' named entity 'DIFFERENT' named entity 'APPROACHES' named entity 'GLYCOSIDES' covid:arg/f4cd52975e6aa33e8c947082eda9b261952b0f8f named entity 'ESCHERICHIA COLI' named entity 'INCREASING' named entity 'PLANT METABOLITES' named entity 'BACKGROUND' named entity 'ESCHERICHIA COLI' named entity 'PRODUCTION' named entity 'DISPLAY' named entity 'ACTIVE' named entity 'QUERCETIN' named entity 'METABOLIC ENGINEERING' named entity 'GLYCOSYLATION' named entity 'OCCUR' named entity 'FLAVONOIDS' named entity 'THESE' named entity 'VARIOUS' named entity 'USE' named entity 'ACTIVE' named entity 'CATALYST' named entity 'PRODUCTION' named entity 'GLYCOSYLATION' named entity 'PROCESSES' named entity 'DUE TO' named entity 'RATES' named entity 'LARGE' named entity 'SPECIALIZED' named entity 'GLYCOSIDES' named entity 'SCALE' named entity 'DEMAND' named entity 'LOW' named entity 'MICROBIAL' named entity 'MARKET' named entity 'HAVE' named entity 'RENDER' named entity 'APPLICATIONS' named entity 'flavonoids' named entity 'titers' named entity 'bio-active' named entity 'catalyst' named entity 'biotechnological' named entity 'Escherichia coli' named entity 'microbial' named entity 'Flavonoids' named entity 'stereospecific' named entity 'glycosylation' named entity 'glycosides' named entity 'Genbank' named entity 'Escherichia coli' named entity 'molecule' named entity 'glycosylated' named entity 'microbial cell factories' named entity 'glycosylation' named entity 'synthetic biology' named entity 'rhamnoside' named entity 'rate-limiting' named entity 'sucrose' named entity 'locus' named entity 'formic acid' named entity 'flavonols' named entity 'glycoside' named entity 'minimal medium' named entity 'plasmids' named entity 'myricetin' named entity 'carbon source' named entity 'antibiotics' named entity 'E. coli' named entity 'glucose' named entity 'stereoselective' named entity 'agarose' named entity 'glycosides' named entity 'quercetin' named entity 'm/z'

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