About: Interaction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems

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About: Interaction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Interaction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems
Creator	Zheng, Bo-Jian Wen, Yu-Mei Wang, Yong-Xiang Xu, A Ae, Jin-Yan Ae, Lin Ae, Poon Chen, J.-Y Chen, W.-N Chen, Wan-Nan Lin, Á Poon, K.-M Vincent, Ae Kwok-Man Wang, Y.-X Wen, Y.-M Zheng, B.-J
Source	Medline; PMC
abstract	To reveal the putative cellular factors involved in SARS coronavirus replication, the helicase (Hel, nsp13) of SARS coronavirus was used to screen the cDNA library of rat pulmonary epithelial cells using the yeast two-hybrid system. Positively interacting proteins were further tested using a mammalian cell hybrid system and co-immunoprecipitation in the human A549 cell line, which has been shown to support SARS coronavirus replication. Out of the seven positive clones observed by yeast two-hybrid assay, only the Ddx5 (Asp-Glu-Ala-Asp box polypeptide 5) protein showed specific interaction with SARS-CoV helicase. When expression of DdX5 was knocked down by small interfering RNA (siRNA), SARS coronavirus replication was significantly inhibited in fetal rhesus kidney (FRhK-4) cells. Since Ddx5 is a multifunctional protein that plays important roles in transcriptional regulation, its interaction with SARS coronavirus helicase provides interesting clues for studying virus–host cell interactions in SARS-CoV infections. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00705-009-0323-y) contains supplementary material, which is available to authorized users.
has issue date	2009-02-18(xsd:dateTime)
bibo:doi	10.1007/s00705-009-0323-y
bibo:pmid	19224332
has license	no-cc
sha1sum (hex)	f59585f68d546ea727e4e1f213a218fa16da0054
schema:url	https://doi.org/10.1007/s00705-009-0323-y
resource representing a document's title	Interaction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems
has PubMed Central identifier	PMC7087151
has PubMed identifier	19224332
schema:publication	Arch Virol
resource representing a document's body	covid:f59585f68d546ea727e4e1f213a218fa16da0054#body_text
is schema:about of	named entity 'knocked' named entity 'clues' named entity 'protein' named entity 'helicase' named entity 'SARS-CoV' named entity 'systems' named entity 'INTERACTION' named entity 'PROTEIN ' named entity 'SARS CORONAVIRUS' named entity 'CLONES' named entity 'MAMMALIAN CELL' named entity 'INFECTIONS' named entity 'INVOLVED' named entity 'INTERACTIONS' named entity 'GLU-ALA' named entity 'PROTEINS' named entity 'USING' named entity 'RAT' named entity 'SPECIFIC' named entity 'SARS-COV' named entity 'CELLS' named entity 'INHIBITED' named entity 'SCREEN' named entity 'CELLULAR' named entity 'TO SUPPORT' named entity 'VIRUS' named entity 'TWO-HYBRID' named entity 'HELICASE' named entity 'CDNA LIBRARY' named entity 'CO-IMMUNOPRECIPITATION' named entity 'PROTEIN ' named entity 'SYSTEMS' named entity 'YEAST' named entity 'INTERACTION' named entity 'MAMMALIAN CELL' named entity 'A549 CELL LINE' named entity 'HELICASE' named entity 'HOST CELL' named entity 'TESTED' named entity 'TRANSCRIPTIONAL REGULATION' named entity 'HUMAN' named entity 'YEAST TWO-HYBRID SYSTEM' named entity 'POLYPEPTIDE' named entity 'PLAYS' named entity 'CELLULAR' named entity 'ALA-ASP' named entity 'PULMONARY' named entity 'KIDNEY' named entity 'ITS' named entity 'INTERESTING' named entity 'SMALL INTERFERING RNA' named entity 'REVEAL' named entity 'IMPORTANT' named entity 'BOX' named entity 'EPITHELIAL CELLS' named entity 'SYSTEM' named entity 'INTERACTING' named entity 'RHESUS' named entity 'ROLES' named entity 'HYBRID' named entity 'PROVIDES' named entity 'POSITIVE' named entity 'EXPRESSION' named entity 'FETAL' named entity 'USED' named entity 'SARS-COV'

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