About: In Vitro Antiviral Activity of Circular Triple Helix Forming Oligonucleotide RNA towards Feline Infectious Peritonitis Virus Replication

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About: In Vitro Antiviral Activity of Circular Triple Helix Forming Oligonucleotide RNA towards Feline Infectious Peritonitis Virus Replication Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	In Vitro Antiviral Activity of Circular Triple Helix Forming Oligonucleotide RNA towards Feline Infectious Peritonitis Virus Replication
Creator	Mehrbod, Parvaneh Choong, Oi Rahman Omar, Abdul Ario Tejo, Bimo
Source	Medline; PMC
abstract	Feline Infectious Peritonitis (FIP) is a severe fatal immune-augmented disease in cat population. It is caused by FIP virus (FIPV), a virulent mutant strain of Feline Enteric Coronavirus (FECV). Current treatments and prophylactics are not effective. The in vitro antiviral properties of five circular Triple-Helix Forming Oligonucleotide (TFO) RNAs (TFO1 to TFO5), which target the different regions of virulent feline coronavirus (FCoV) strain FIPV WSU 79-1146 genome, were tested in FIPV-infected Crandell-Rees Feline Kidney (CRFK) cells. RT-qPCR results showed that the circular TFO RNAs, except TFO2, inhibit FIPV replication, where the viral genome copy numbers decreased significantly by 5-fold log(10) from 10(14) in the virus-inoculated cells to 10(9) in the circular TFO RNAs-transfected cells. Furthermore, the binding of the circular TFO RNA with the targeted viral genome segment was also confirmed using electrophoretic mobility shift assay. The strength of binding kinetics between the TFO RNAs and their target regions was demonstrated by NanoITC assay. In conclusion, the circular TFOs have the potential to be further developed as antiviral agents against FIPV infection.
has issue date	2014-02-20(xsd:dateTime)
bibo:doi	10.1155/2014/654712
bibo:pmid	24707494
has license	cc-by
sha1sum (hex)	f5ad2323eb387f6e271e2842bb2cc4a33504fde3
schema:url	https://doi.org/10.1155/2014/654712
resource representing a document's title	In Vitro Antiviral Activity of Circular Triple Helix Forming Oligonucleotide RNA towards Feline Infectious Peritonitis Virus Replication
has PubMed Central identifier	PMC3950953
has PubMed identifier	24707494
schema:publication	Biomed Res Int
resource representing a document's body	covid:f5ad2323eb387f6e271e2842bb2cc4a33504fde3#body_text
is schema:about of	named entity 'cells' named entity 'infection' named entity 'feline coronavirus' named entity 'FIPV' named entity 'Feline' covid:arg/f5ad2323eb387f6e271e2842bb2cc4a33504fde3 named entity 'RNAs' named entity 'binding' named entity 'RT-qPCR' named entity 'TFO' named entity 'electrophoretic mobility shift assay' named entity 'genome' named entity 'Coronavirus' named entity 'copy numbers' named entity 'WSU' named entity 'virus' named entity 'virulent' named entity 'kinetics' named entity 'TFO' named entity 'FCoV' named entity 'Current treatments' named entity 'virus' named entity 'FIP' named entity 'assay' named entity 'Triple Helix' named entity 'FIPV' named entity 'single stranded' named entity 'double helical' named entity 'transfected' named entity 'FIPV' named entity 'Hoogsteen' named entity 'post hoc test' named entity 'transfected' named entity 'quantitative real-time PCR' named entity 'Triple Helix' named entity 'TFO' named entity 'virus replication' named entity 'serotype' named entity '4.03' named entity 'FIPV' named entity 'psoralen' named entity 'FIPV' named entity 'EMSA' named entity 'TFO' named entity 'urea' named entity 'antiviral therapies' named entity 'FIPV' named entity 'TFO' named entity 'virus' named entity 'positive and negative controls' named entity 'copy numbers' named entity 'Qiagen' named entity '96-well plate' named entity 'virus replication' named entity 'Open Reading Frames' named entity 'TFOs' named entity 'infection' named entity 'transfection' named entity 'TFO' named entity 'antiviral activity' named entity 'cancer cells' named entity 'H1N1' named entity 'ATCC' named entity 'intracellular' named entity 'collagen' named entity 'FIPV' named entity 'Watson-Crick' named entity 'polyacrylamide gel' named entity 'RNA' named entity 'TFO' named entity 'transfection' named entity 'TFO' named entity 'virulent' named entity 'hybridization' named entity 'siRNA' named entity 'transfection' named entity 'TFO' named entity 'hydrogen bonds'

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