About: Generation of stable monoclonal antibody-producing BCR(+) human memory B cells by genetic programming

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About: Generation of stable monoclonal antibody-producing BCR(+) human memory B cells by genetic programming Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Generation of stable monoclonal antibody-producing BCR(+) human memory B cells by genetic programming
Creator	Beaumont, Tim Spits, Hergen Yasuda, Etsuko Bakker, Arjen Bogers, Willy Diehl, Sean Kwakkenbos, Mark Lukens, Michaël Mei, Henrik Radbruch, Andreas Scheeren, Ferenc Van Bleek, Grada Van Geelen, Caroline Widjojoatmodjo, Myra
Source	Medline; PMC
abstract	B cell lymphoma (BCL)6 and Bcl-xL are expressed in germinal center (GC) B cells and enable them to endure the proliferative and mutagenic environment of the GC. By introducing these genes into peripheral blood memory B cells and culturing these cells with factors produced by follicular helper T cells, CD40L and IL-21, we convert them to highly proliferating, cell surface BCR positive, Ig-secreting B cells with features of GC B cells including expression of activation-induced cytidine deaminase. We generated cloned lines of B cells specific for respiratory syncytial virus and used these cells as a source of antibodies that effectively neutralized this virus in vivo. This method provides a new tool to study GC B cell biology, signal transduction through antigen-specific B cell receptors, and for the rapid generation of high affinity human monoclonal antibodies.
has issue date	2009-12-20(xsd:dateTime)
bibo:doi	10.1038/nm.2071
bibo:pmid	20023635
has license	no-cc
sha1sum (hex)	f5f5f2c5e7570a4152e6bbdecada50edc34a786d
schema:url	https://doi.org/10.1038/nm.2071
resource representing a document's title	Generation of stable monoclonal antibody-producing BCR(+) human memory B cells by genetic programming
has PubMed Central identifier	PMC2861345
has PubMed identifier	20023635
schema:publication	Nat Med
resource representing a document's body	covid:f5f5f2c5e7570a4152e6bbdecada50edc34a786d#body_text
is schema:about of	named entity 'MONOCLONAL ANTIBODY' named entity 'MEMORY B CELLS' named entity '1-2' named entity 'GENERATION' named entity 'REFERENCE' named entity 'PRODUCING' named entity 'METHODS' named entity 'HUMAN' named entity 'FIGURES' named entity 'GENETIC PROGRAMMING' named entity 'BCR' named entity 'STABLE' named entity 'monoclonal antibody' named entity 'BCR' named entity 'genetic programming' named entity 'memory B cells'
is part of of	Generation of stable monoclonal antibody-producing BCR(+) human memory B cells by genetic programming B cell lymphoma (BCL)6 and Bcl-xL are expressed in germinal center (GC) B cells and enable them to endure the proliferative and mutagenic environment of the GC. By introducing these genes into peripheral blood memory B cells and culturing these cells with factors produced by follicular helper T cells, CD40L and IL-21, we convert them to highly proliferating, cell surface BCR positive, Ig-secreting B cells with features of GC B cells including expression of activation-induced cytidine deaminase. We generated cloned lines of B cells specific for respiratory syncytial virus and used these cells as a source of antibodies that effectively neutralized this virus in vivo. This method provides a new tool to study GC B cell biology, signal transduction through antigen-specific B cell receptors, and for the rapid generation of high affinity human monoclonal antibodies. covid:f5f5f2c5e7570a4152e6bbdecada50edc34a786d#body_text

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