About: Exchange of amino acids in the H1-haemagglutinin to H3 residues is required for efficient influenza A virus replication and pathology in Tmprss2 knock-out mice

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About: Exchange of amino acids in the H1-haemagglutinin to H3 residues is required for efficient influenza A virus replication and pathology in Tmprss2 knock-out mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Exchange of amino acids in the H1-haemagglutinin to H3 residues is required for efficient influenza A virus replication and pathology in Tmprss2 knock-out mice
Creator	Anhlan, Darisuren Gerhauser, Ingo Kollmus, Heike Krausze, Joern Kühn, Nora Pippel, Jan Schughart, Klaus Hrincius, Eike Lambertz, Ruth
Source	Medline; PMC
abstract	The haemagglutinin (HA) of H1N1 and H3N2 influenza A virus (IAV) subtypes has to be activated by host proteases. Previous studies showed that H1N1 virus cannot replicate efficiently in Tmprss2(−/−) knock-out mice whereas H3N2 viruses are able to replicate to the same levels in Tmprss2(−/−) as in wild type (WT) mice. Here, we investigated the sequence requirements for the HA molecule that allow IAV to replicate efficiently in the absence of TMPRSS2. We showed that replacement of the H3 for the H1-loop sequence (amino acids 320 to 329, at the C-terminus of HA(1)) was not sufficient for equal levels of virus replication or severe pathology in Tmprss2(−/−) knock-out mice compared to WT mice. However, exchange of a distant amino acid from H1 to H3 sequence (E31D) in addition to the HA-loop substitution resulted in virus replication in Tmprss2(−/−) knock-out mice that was comparable to WT mice. The higher virus replication and lung damage was associated with increased epithelial damage and higher mortality. Our results provide further evidence and insights into host proteases as a promising target for therapeutic intervention of IAV infections.
has issue date	2018-08-06(xsd:dateTime)
bibo:doi	10.1099/jgv.0.001128
bibo:pmid	30084768
has license	cc-by
sha1sum (hex)	f62e14e75bd446217bb09ec6824559f88116c366
schema:url	https://doi.org/10.1099/jgv.0.001128
resource representing a document's title	Exchange of amino acids in the H1-haemagglutinin to H3 residues is required for efficient influenza A virus replication and pathology in Tmprss2 knock-out mice
has PubMed Central identifier	PMC6230768
has PubMed identifier	30084768
schema:publication	J Gen Virol
resource representing a document's body	covid:f62e14e75bd446217bb09ec6824559f88116c366#body_text
is schema:about of	named entity 'subtypes' named entity 'mice' named entity 'studies' named entity 'compared' named entity 'levels' named entity 'Tmprss2' named entity 'knock-out mice' named entity 'MICE' named entity 'HAEMAGGLUTININ' named entity 'EVIDENCE' named entity 'INFLUENZA A VIRUS' named entity 'SEQUENCE' named entity 'SEVERE' named entity 'EXCHANGE' named entity 'REPLACEMENT' named entity 'REQUIREMENTS' named entity 'LOOP' named entity 'ADDITION' named entity 'EPITHELIAL DAMAGE' named entity 'VIRUSES' named entity 'HOST' named entity 'ACIDS' named entity 'VIRUS REPLICATION' named entity 'C-TERMINUS' named entity 'PROTEASES' named entity 'MORTALITY' named entity 'LEVELS' named entity 'HIGHER' named entity 'DAMAGE' named entity 'HERE' named entity '320' named entity 'MOLECULE' named entity 'SUFFICIENT' named entity 'AMINO ACID' named entity '80%' named entity 'INSIGHTS' named entity 'TMPRSS2' named entity 'PATHOLOGY' named entity 'AMINO ACIDS' named entity 'HAEMAGGLUTININ' named entity 'ASSOCIATED WITH' named entity 'INCREASED' named entity 'REQUIRED' named entity 'VIRUS REPLICATION' named entity 'EFFICIENT' named entity 'INFLUENZA A VIRUS' named entity 'EXCHANGE' named entity 'KNOCK-OUT' named entity 'PATHOLOGY' named entity 'THERAPEUTIC INTERVENTION' named entity 'EQUAL' named entity 'INVESTIGATED' named entity 'LUNG' named entity 'H1N1' named entity 'PREVIOUS' named entity 'KNOCK-OUT' named entity 'TO BE ACTIVATED' named entity 'STUDIES' named entity 'VIRUS' named entity 'KNOCKOUT MICE' named entity 'INFECTIONS' named entity 'MICE' named entity 'TARGET' named entity 'COMPARED' named entity 'ABSENCE OF' named entity 'SUBTYPES' named entity 'WILD TYPE' named entity 'H3N2 INFLUENZA' named entity 'RESULTS' named entity 'REPLICATE' named entity 'TMPRSS2' named entity 'H3N2' named entity 'PROVIDE'

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