About: Immunogenicity analysis of conserved fragments in Plasmodium ovale species merozoite surface protein 4

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About: Immunogenicity analysis of conserved fragments in Plasmodium ovale species merozoite surface protein 4 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Immunogenicity analysis of conserved fragments in Plasmodium ovale species merozoite surface protein 4
Creator	Cao, Jun Cheng, Yang Chu, Ruilin Fu, Haitian Kassegne, Kokouvi Lei, Yao Shen, Feihu Sun, Yifan Uwase, Juliette Xuan, Yinghua
Source	PMC
abstract	BACKGROUND: There is an urgent need for an effective vaccine to control and eradicate malaria, one of the most serious global infectious diseases. Plasmodium merozoite surface protein 4 (MSP4) has been listed as a blood-stage subunit vaccine candidate for malaria. Infection with Plasmodium ovale species including P. ovale wallikeri and P. ovale curtisi, is also a source of malaria burden in tropical regions where it is sometimes mixed with other Plasmodium species. However, little is known about P. ovale MSP4. METHODS: The msp4 gene was amplified through polymerase chain reaction using genomic DNA extracted from blood samples of 46 patients infected with P. ovale spp. and amplified products were sequenced. Open reading frames predicted as immunogenic peptides consisting of 119 and 97 amino acids of P. ovale curtisi MSP4 (PocMSP4) and P. ovale wallikeri MSP4 (PowMSP4), respectively, were selected for protein expression. Recombinant proteins (rPoMSP4) were expressed in Escherichia coli, purified, analysed, and immunized in BALB/c mice. The specificity of anti-MSP4-immunoglobulin (Ig) G antibodies was evaluated by Western blot and enzyme-linked immunosorbent assays, and cellular immune responses were analysed via lymphocyte proliferation assays. RESULTS: Full peptide sequences of PocMSP4 and PowMSP4 were completely conserved in all clinical isolates, except in the epidermal growth factor-like domain at the carboxyl terminus where only one mutation was observed in one P. o. wallikeri isolate. Further, truncated PoMSP4 segments were successfully expressed and purified as ~ 32 kDa proteins. Importantly, high antibody responses with end-point titres ranging from 1:10,000 to 1:2,560,000 in all immunized mouse groups were observed, with high IgG avidity to PocMSP4 (80.5%) and PowMSP4 (92.3%). Furthermore, rPocMSP4 and rPowMSP4 cross-reacted with anti-PowMSP4-specific or anti-PocMSP4-specific antibodies. Additionally, anti-PoMSP4 IgG antibodies showed broad immuno-specificity in reacting against rPoMSP1 and rPoAMA1. Lastly, PocMSP4- and PowMSP4-immunized mice induced cellular immune responses with PocMSP4 (36%) and PowMSP4 cells (15.8%) during splenocyte proliferation assays. CONCLUSION: Findings from this study suggest conservation in PoMSP4 protein sequences and high immunogenicity was observed in rPoMSP4. Furthermore, induction of immune responses in PocMSP4- and PowMSP4-immunized mice informed that both humoral and cellular immune responses play crucial roles for PoMSP4 in protection.
has issue date	2020-03-30(xsd:dateTime)
bibo:doi	10.1186/s12936-020-03207-7
bibo:pmid	32228600
has license	cc-by
sha1sum (hex)	f6db034174b9ff4bba246040650d30533d1e2e56
schema:url	https://doi.org/10.1186/s12936-020-03207-7
resource representing a document's title	Immunogenicity analysis of conserved fragments in Plasmodium ovale species merozoite surface protein 4
has PubMed Central identifier	PMC7106901
has PubMed identifier	32228600
schema:publication	Malar J
resource representing a document's body	covid:f6db034174b9ff4bba246040650d30533d1e2e56#body_text
is schema:about of	named entity 'protein' named entity 'P. ovale' named entity 'BACKGROUND' named entity 'KNOWN' named entity 'MIXED' named entity 'global' named entity 'eradicate' named entity 'species' named entity 'burden' named entity 'Plasmodium ovale' named entity 'protein' named entity 'P. ovale' named entity 'P. ovale curtisi' named entity 'Infection' named entity 'P. ovale wallikeri' named entity 'MSP1' named entity 'immunogenicity' named entity 'asexual' named entity 'enzyme-linked immunosorbent assays' named entity 'merozoite' named entity 'malaria parasites' named entity 'cross-reactivity' named entity 'immune response' named entity 'gene' named entity 'Western blot' named entity 'antigens' named entity 'erythrocytic' named entity 'Coomassie brilliant blue' named entity 'BSA' named entity 'P. vivax' named entity 'antibody' named entity 'PlasmoDB' named entity 'immune responses' named entity 'antigen' named entity 'immune responses' named entity 'malaria' named entity 'sonication' named entity 'nucleotide' named entity 'IgG antibody' named entity 'Cross-reactivity' named entity 'Lymphocyte' named entity 'epidermal growth factor' named entity 'P. falciparum' named entity 'PBS' named entity 'goat' named entity 'malaria' named entity 'positive control' named entity 'malaria' named entity 'antibodies' named entity 'cloned' named entity 'antigens' named entity 'PCR' named entity 'splenocyte' named entity 'febrile' named entity 'centrifugation' named entity 'antibody' named entity 'cross-reaction' named entity 'Tris-HCl' named entity 'malaria parasites' named entity 'goat' named entity 'Cross-reaction' named entity 'murine' named entity 'immune responses' named entity 'World Health Organization' named entity 'antigen' named entity 'imidazole' named entity 'ORFs' named entity 'DTT' named entity 'P. yoelii' named entity 'cellular immune response' named entity 'humoral responses' named entity 'Antigens'

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