About: Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification

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About: Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification
Creator	Han, Cheng-Gui Li, Da-Wei Liu, Xiao-Jun Yu, Jia-Lin Zhang, Zong-Ying
Source	Medline; PMC
abstract	For the detection of wheat yellow mosaic virus (WYMV), we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method. Using Primer Explorer software, four sets of primers were designed and RT-LAMP assay reaction conditions were optimized. The RT-LAMP was performed at different times by four primer sets. Agarose gel analysis showed that WYMV could be detected after 30 min with the primer set III and after 45 min with the other three primer sets, both under the 80-min reaction time. RT-LAMP had the same results with the four primer sets, thus primer set III and 65°C for 80 min reaction were selected for virus detection. There was no significant different when avian myeloblastosis virus (AMV) and moloney murine leukemia virus (M-MLV) RT-LAMP with the four primer sets and M-MLV was chosen due to its relatively cheap price. The result on specificity showed that the assay could amplify WYMV specifically, and the sensitivity comparison showed that the RT-LAMP was 100 times more sensitive than conventional reverse-transcriptase-polymerase chain reaction (RT-PCR). Overall, RT-LAMP was found to be a simple, specific, sensitive, convenient and time-saving method for WYMV detection.
has issue date	2011-12-20(xsd:dateTime)
bibo:doi	10.1186/1743-422x-8-550
bibo:pmid	22185375
has license	cc-by
sha1sum (hex)	f85f1c88d5f92b9177f3c652fe643d7f13df947e
schema:url	https://doi.org/10.1186/1743-422x-8-550
resource representing a document's title	Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification
has PubMed Central identifier	PMC3260119
has PubMed identifier	22185375
schema:publication	Virol J
resource representing a document's body	covid:f85f1c88d5f92b9177f3c652fe643d7f13df947e#body_text
is schema:about of	named entity 'primer' named entity 'sets' named entity 'sensitive' named entity 'chosen' named entity 'ITS' named entity 'SPECIFICITY' named entity 'SPECIFIC' named entity 'TIMES' named entity 'RESULTS' named entity 'POLYMERASE CHAIN REACTION' named entity 'MOLONEY MURINE LEUKEMIA VIRUS' named entity 'PRIMER' named entity '30 MIN' named entity 'MLV' named entity 'RESULT' named entity '100' named entity 'COMPARISON' named entity 'REACTION TIME' named entity 'RT-LAMP ASSAY' named entity 'ESTABLISHED' named entity 'AMPLIFY' named entity 'SOFTWARE' named entity 'DIFFERENT' named entity 'USING' named entity 'CONVENIENT' named entity 'SET' named entity 'REVERSE-TRANSCRIPTASE' named entity 'VIRUS DETECTION' named entity 'REVERSE TRANSCRIPTION' named entity 'METHOD' named entity 'SENSITIVE' named entity 'REACTION' named entity 'AVIAN MYELOBLASTOSIS VIRUS' named entity 'COULD BE' named entity 'III' named entity 'EXPLORER' named entity 'WHEAT YELLOW MOSAIC VIRUS' named entity 'DETECTION' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'RAPID' named entity 'REVERSE TRANSCRIPTION' named entity 'DETECTED' named entity 'PCR' named entity 'CHOSEN' named entity 'SELECTED' named entity 'TIME' named entity 'ASSAY' named entity 'LAMP' named entity 'PRIMERS' named entity 'DUE TO' named entity 'PERFORMED' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'CONVENTIONAL' named entity 'SENSITIVITY' named entity 'OVERALL' named entity 'MIN' named entity 'SIMPLE' named entity 'PRICE' named entity 'WHEAT YELLOW MOSAIC VIRUS' named entity 'FOUND' named entity 'AGAROSE GEL ANALYSIS' named entity 'CONDITIONS' named entity 'DETECTION' named entity 'SIGNIFICANT' covid:arg/f85f1c88d5f92b9177f3c652fe643d7f13df947e named entity 'sensitivity' named entity 'reaction time' named entity 'Primer' named entity 'primer' named entity 'set' named entity 'The' named entity 'specificity' named entity 'virus' named entity 'primer' named entity 'primer' named entity 'Agarose gel' named entity 'assay'

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