About: ViralORFeome: an integrated database to generate a versatile collection of viral ORFs

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: ViralORFeome: an integrated database to generate a versatile collection of viral ORFs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	ViralORFeome: an integrated database to generate a versatile collection of viral ORFs
Creator	Achaz, G Andre, P Aublin-Gex, A Caignard, G Cassonnet, P Chaboud, A Chantier, T Combet, C Deleage, G Delmotte, S Deloire, A Demeret, C Favre, M Gautier, C Guironnet-Paquet, A Jacob, Y Jacotot, L Le Breton, M Lotteau, V Lucas-Hourani, M Meyniel, L Navratil, V Neveu, G Pellet, J Rabourdin-Combe, C Tafforeau, L Tangy, F Vaglio, P Vidalain, P
Source	PMC
abstract	Large collections of protein-encoding open reading frames (ORFs) established in a versatile recombination-based cloning system have been instrumental to study protein functions in high-throughput assays. Such ‘ORFeome’ resources have been developed for several organisms but in virology, plasmid collections covering a significant fraction of the virosphere are still needed. In this perspective, we present ViralORFeome 1.0 (http://www.viralorfeome.com), an open-access database and management system that provides an integrated set of bioinformatic tools to clone viral ORFs in the Gateway® system. ViralORFeome provides a convenient interface to navigate through virus genome sequences, to design ORF-specific cloning primers, to validate the sequence of generated constructs and to browse established collections of virus ORFs. Most importantly, ViralORFeome has been designed to manage all possible variants or mutants of a given ORF so that the cloning procedure can be applied to any emerging virus strain. A subset of plasmid constructs generated with ViralORFeome platform has been tested with success for heterologous protein expression in different expression systems at proteome scale. ViralORFeome should provide our community with a framework to establish a large collection of virus ORF clones, an instrumental resource to determine functions, activities and binding partners of viral proteins.
has issue date	2009-12-08(xsd:dateTime)
bibo:doi	10.1093/nar/gkp1000
bibo:pmid	20007148
has license	cc-by-nc
sha1sum (hex)	f87d2665175ce7e1c6ffd14e0da7bfc21b784e2e
schema:url	https://doi.org/10.1093/nar/gkp1000
resource representing a document's title	ViralORFeome: an integrated database to generate a versatile collection of viral ORFs
has PubMed Central identifier	PMC2808970
has PubMed identifier	20007148
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:f87d2665175ce7e1c6ffd14e0da7bfc21b784e2e#body_text
is schema:about of	named entity 'VIRAL' named entity 'DATABASE' named entity 'COLLECTION' named entity 'INTEGRATED' named entity 'ORFS' named entity 'GENERATE' named entity 'integrated database' named entity 'subcellular localization' named entity 'pathogenesis' named entity 'contig' named entity 'Phred' named entity 'virus' named entity 'proteomic' named entity 'poliovirus' named entity 'antiviral' named entity 'virus' named entity 'virus species' named entity 'HCV' named entity 'cloned' named entity 'IFN' named entity 'plasmid' named entity 'virus' named entity 'cDNA clone' named entity 'menu bar' named entity 'wild-type virus' named entity 'menu bar' named entity 'ORF' named entity 'virulence' named entity 'primers' named entity 'viruses' named entity 'Semliki forest' named entity 'cloning' named entity 'clone' named entity 'nucleus' named entity 'genome' named entity 'bioinformatic tools' named entity 'TNF' named entity 'ORFs' named entity 'viral RNA' named entity 'mutation' named entity 'NF-kB' named entity 'genomic sequence' named entity 'Sindbis' named entity 'vector' named entity 'type I interferon' named entity 'IFN' named entity 'cloning' named entity 'primers' named entity 'virus' named entity 'ORFs' named entity 'virology' named entity 'virus' named entity 'replicons'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software