About: Nocardia polymerase chain reaction (PCR)-based assay performed on bronchoalveolar lavage fluid after lung transplantation: A prospective pilot study

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About: Nocardia polymerase chain reaction (PCR)-based assay performed on bronchoalveolar lavage fluid after lung transplantation: A prospective pilot study Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Nocardia polymerase chain reaction (PCR)-based assay performed on bronchoalveolar lavage fluid after lung transplantation: A prospective pilot study
Creator	Steensels, Deborah Bergeron, Emmanuelle Claes, Vincent El Bizri, Najla Kohnen, Michel Lebeaux, David Rodriguez-Nava, Veronica Coussement Id, Julien Lène Salord, Hé Tienne, Isabelle
Source	Medline; PMC
abstract	BACKGROUND: Transplant recipients are at risk of pulmonary nocardiosis, a life-threatening opportunistic infection caused by Nocardia species. Given the limitations of conventional diagnostic techniques (i.e., microscopy and culture), a polymerase chain reaction (PCR)-based assay was developed to detect Nocardia spp. on clinical samples. While this test is increasingly being used by transplant physicians, its performance characteristics are not well documented. We evaluated the performance characteristics of this test on bronchoalveolar lavage (BAL) fluid samples from lung transplant recipients (LTRs). METHODS: We prospectively included all BAL samples from LTRs undergoing bronchoscopy at our institution between December 2016 and June 2017 (either surveillance or clinically-indicated bronchoscopies). Presence of microbial pathogens was assessed using techniques available locally (including microscopy and 10-day culture for Nocardia). BAL samples were also sent to the French Nocardiosis Observatory (Lyon, France) for the Nocardia PCR-based assay. Transplant physicians and patients were blinded to the Nocardia PCR results. RESULTS: We included 29 BAL samples from 21 patients (18 surveillance and 11 clinically-indicated bronchoscopies). Nocardiosis was not diagnosed in any of these patients by conventional techniques. However, Nocardia PCR was positive in five BAL samples from five of the patients (24%, 95% confidence interval: 11–45%); four were asymptomatic and undergoing surveillance bronchoscopy, and one was symptomatic and was later diagnosed with influenza virus infection. None of the five PCR-positive patients died or were diagnosed with nocardiosis during the median follow-up of 21 months after the index bronchoscopy (range: 20–23 months). CONCLUSIONS: In this prospective study, Nocardia PCR was positive on BAL fluid from one fourth of the LTRs. Nocardia PCR-based assays should be used with caution on respiratory samples from LTRs because of the possible detection of airway colonization using this technique. Larger studies are required to determine the usefulness of the Nocardia PCR-based assay in transplant recipients.
has issue date	2019-02-25(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0211989
bibo:pmid	30802260
has license	cc-by
sha1sum (hex)	f8e8d36bf5ce0686ed639aceace213100fdba042
schema:url	https://doi.org/10.1371/journal.pone.0211989
resource representing a document's title	Nocardia polymerase chain reaction (PCR)-based assay performed on bronchoalveolar lavage fluid after lung transplantation: A prospective pilot study
has PubMed Central identifier	PMC6388935
has PubMed identifier	30802260
schema:publication	PLoS One
resource representing a document's body	covid:f8e8d36bf5ce0686ed639aceace213100fdba042#body_text
is schema:about of	named entity 'PCR' named entity 'Transplant' named entity 'chain reaction' named entity 'lung transplant' named entity 'risk' named entity 'opportunistic infection' named entity 'PCR' named entity 'assay' named entity 'Nocardia' named entity 'heat shock protein' named entity 'microorganisms' named entity 'Lung transplant' named entity 'Nocardia' named entity 'Nocardia' named entity 'Nocardia' named entity 'inhalation' named entity 'PCR' named entity 'bronchoscopy' named entity 'cotrimoxazole' named entity 'immunosuppressive drugs' named entity 'nocardiosis' named entity 'nocardiosis' named entity 'dyspnea' named entity 'culture results' named entity 'amoxicillin-clavulanate' named entity 'PCR' named entity 'Nocardia' named entity 'Mycobacterium tuberculosis' named entity 'bronchoscopy' named entity 'Transbronchial' named entity 'nocardiosis' named entity 'single center' named entity 'biopsy' named entity 'sequence similarity' named entity 'sheep' named entity 'Pneumocystis jirovecii' named entity 'asymptomatic' named entity 'pulmonary infections' named entity 'PCR' named entity 'gut microbiota' named entity 'TB12' named entity 'Nocardia' named entity 'nocardiosis' named entity 'Polymyxin' named entity 'nocardiosis' named entity 'autoimmune diseases' named entity 'PCR' named entity 'bacterial pathogens' named entity 'PCR' named entity 'pathogens' named entity 'GenBank' named entity 'molecular level' named entity 'lung transplant' named entity 'CMV' named entity 'Clostridium difficile-associated diarrhea' named entity 'mucociliary clearance' named entity 'antigen' named entity '16S rRNA' named entity 'clinical practice' named entity '95% confidence interval' named entity 'cotrimoxazole' named entity 'Lyon' named entity 'PCR' named entity 'Becton Dickinson' named entity '16S rRNA' named entity 'Nocardia infection' named entity 'PCR-based' named entity 'Toxoplasma gondii' named entity 'PCR amplification' named entity 'nocardiosis' named entity 'antibiotics' named entity 'follow-up'

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