About: Gastric gross and microscopic lesions caused by the UNAM-97 variant strain of infectious bronchitis virus after the eighth passage in specific pathogen-free chicken embryos

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About: Gastric gross and microscopic lesions caused by the UNAM-97 variant strain of infectious bronchitis virus after the eighth passage in specific pathogen-free chicken embryos Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Gastric gross and microscopic lesions caused by the UNAM-97 variant strain of infectious bronchitis virus after the eighth passage in specific pathogen-free chicken embryos
Creator	Escorcia, M Galindo, F Fortoul, T Ló Pez, C Petrone, V Téllez, G
Source	Elsevier; Medline; PMC
abstract	Abstract Herein we report a description of gross and microscopic lesions found in specific pathogen-free chicken embryos caused by UNAM-97 infectious bronchitis virus (IBV) variant strain after the eighth passage. Embryos were divided into three groups and were inoculated in the chorioallantoic sac with 0.2 mL of UNAM-97, Mass 41 IBV (positive control), or sterile PBS (negative control). Forty-eight hours later the allatoic fluid was taken and used to start a cycle of eight passages through 9-d-old embryos. Seven days after the last passage, embryos were harvested and macroscopic lesions in all organs were recorded. Proventriculus and gizzard samples were obtained from all embryos and routinely processed for microscopic and ultrastructural examinations. The UNAM-97 IBV variant strain caused two macroscopic lesions uncommon for Mexican strains: thin-walled proventriculus and gizzard, as well as urate accumulation within an extra-embryonic peritoneal sac, leaving the body through the umbilical duct and accompanied by the yolk sac. At microscopic level, two relevant findings were observed to be produced by this variant. In the proventriculus, there was a decrease in the gland papillary branching, while the gizzard showed a significant reduction in mucosa thickness and tubular-to-proliferative-cell ratio, as well as an absence of hyaline secretion in the lumen. Electrodense material scattered in proventricular and gizzard cells was observed, with a structure consistent with that of coronaviruses. These pathological chicken embryo findings have not been reported as being caused by other IBV strains in Mexico.
has issue date	2002-11-01(xsd:dateTime)
bibo:doi	10.1093/ps/81.11.1647
bibo:pmid	12455591
has license	els-covid
sha1sum (hex)	f942a826a2868fe8acaef4bf2e975c2c21b21af7
schema:url	https://doi.org/10.1093/ps/81.11.1647
resource representing a document's title	Gastric gross and microscopic lesions caused by the UNAM-97 variant strain of infectious bronchitis virus after the eighth passage in specific pathogen-free chicken embryos
has PubMed Central identifier	PMC7107215
has PubMed identifier	12455591
schema:publication	Poultry Science
resource representing a document's body	covid:f942a826a2868fe8acaef4bf2e975c2c21b21af7#body_text
is schema:about of	named entity 'FREE' named entity 'ultrastructural' named entity 'embryos' named entity 'UNAM' named entity 'EMBRYOS' named entity 'REPORT' named entity 'RECORDED' named entity 'EIGHTH' named entity 'START' named entity 'CHICKEN' named entity 'GROSS' named entity 'OLD' named entity 'PROCESSED' named entity 'PATHOGEN' named entity 'FOUND' named entity '0.2' named entity 'PBS' named entity 'DAYS' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'SPECIFIC' named entity 'STRAIN' named entity 'PASSAGE' named entity 'LESIONS' named entity 'ENVIRONMENT' named entity 'HEALTH' named entity 'PROVENTRICULUS' named entity 'TAKEN' named entity 'HOURS' named entity 'MICROSCOPIC' named entity 'SPECIFIC' named entity 'BEHAVIOR' named entity 'EIGHTH' named entity 'CAUSED BY' named entity 'GASTRIC' named entity 'PATHOGEN' named entity 'VARIANT' named entity 'CHICKEN' named entity 'SAMPLES' named entity 'STRAIN' named entity 'GROSS' named entity 'PASSAGE' named entity 'MICROSCOPIC' named entity 'CONTROL' named entity 'ORGANS' named entity 'USED' named entity 'CAUSED BY' named entity 'MACROSCOPIC' named entity 'FORTY-EIGHT' named entity 'EMBRYOS' named entity 'SEVEN' named entity 'DESCRIPTION' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'CYCLE' named entity 'OBTAINED' named entity 'EXAMINATIONS' named entity 'SAC' named entity 'FREE' named entity 'LESIONS' named entity 'FLUID' named entity 'LATER' named entity 'MASS' named entity 'STERILE' named entity 'VARIANT' named entity 'DIVIDED' named entity 'GROUPS' named entity 'GIZZARD' named entity 'eighth' named entity 'days' named entity 'UNAM' named entity 'specific' named entity 'Mass' named entity 'embryos' named entity 'embryos' named entity 'Embryos' named entity 'allantoic' named entity 'gizzard'

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