About: Genetic characteristics, pathogenicity, and immunogenicity associated with cell adaptation of a virulent genotype 2b porcine epidemic diarrhea virus

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Genetic characteristics, pathogenicity, and immunogenicity associated with cell adaptation of a virulent genotype 2b porcine epidemic diarrhea virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Genetic characteristics, pathogenicity, and immunogenicity associated with cell adaptation of a virulent genotype 2b porcine epidemic diarrhea virus
Creator	Lee, Changhee Son, Kyu-Yeol Lee, Seung-Chul Choi, Hwan-Won Noh, Yun-Hee Yoon, In-Joong Lee, Sunhee
Source	Elsevier; Medline; PMC
abstract	Porcine epidemic diarrhea virus (PEDV) has emerged or re-emerged worldwide, posing a significant financial threat to major pig-producing countries. In the present study, a virulent Korean pandemic PEDV strain, KNU-141112, was serially propagated in Vero cells for up to 100 passages. Through cell culture adaptation, we obtained four distinct deletion (DEL) mutants by plaque purification followed by nucleotide sequencing of the spike (S)/ORF3 gene-coding region, which were designated KNU-141112-S DEL2, −S DEL5, −S DEL2/ORF3, and −S DEL5/ORF3. Further whole genome sequencing identified 12 or 14 amino acid changes in the cell-adapted DEL strains. Animal inoculation studies revealed that the virulence of both S DEL2/ORF3 and S DEL5/ORF3 viruses with a large 46-nt deletion in the intergenic portion of S and ORF3 was remarkably diminished, indicating viral attenuation in the natural host. Furthermore, these cell-adapted strains elicited potent neutralizing antibody responses in immunized pigs. Taken together, our data indicate that the cell-attenuated S DEL2/ORF3 and S DEL5/ORF3 strains are promising candidates for the development of a safe and effective live PEDV vaccine.
has issue date	2017-06-28(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2017.06.019
bibo:pmid	28757031
has license	no-cc
sha1sum (hex)	f953b3d21ebfd22f0c0f4bd7f579de50196578f9
schema:url	https://doi.org/10.1016/j.vetmic.2017.06.019
resource representing a document's title	Genetic characteristics, pathogenicity, and immunogenicity associated with cell adaptation of a virulent genotype 2b porcine epidemic diarrhea virus
has PubMed Central identifier	PMC7172462
has PubMed identifier	28757031
schema:publication	Vet Microbiol
resource representing a document's body	covid:f953b3d21ebfd22f0c0f4bd7f579de50196578f9#body_text
is schema:about of	named entity 'live' named entity 'attenuation' named entity 'revealed' named entity 'DEL2' named entity 'Vero' named entity 'DESIGNATED' named entity 'NEUTRALIZING ANTIBODY' named entity 'CHANGES' named entity 'DIMINISHED' named entity 'PRODUCING' named entity 'ATTENUATION' named entity 'REVEALED' named entity 'promising' named entity 'propagated' named entity 'viral' named entity 'potent' named entity 'signal peptide' named entity 'Invitrogen' named entity 'PBS' named entity 'xylene' named entity 'terminator' named entity 'plaque assay' named entity 'efficacy of the vaccine' named entity 'ion channel' named entity 'PEDV' named entity 'lethargy' named entity 'serially diluted' named entity 'strains' named entity 'posing' named entity 'virulence' named entity 'intergenic' named entity 'cell culture' named entity 'coding region' named entity 'DEL' named entity 'pathogenicity' named entity 'genotype' named entity 'Asia' named entity 'Institutional Animal Care and Use Committee' named entity 'immunization' named entity 'PEDV' named entity 'post-mortem examinations' named entity 'Invitrogen' named entity 'viruses' named entity 'next-generation sequencing' named entity 'valine' named entity 'DEL' named entity 'syncytial' named entity 'RT-PCR' named entity 'protein' named entity 'cell formation' named entity 'Difco' named entity 'DEL' named entity 'Asia' named entity 'C-terminal' named entity 'minimum essential medium' named entity 'BSA' named entity '37°C' named entity 'pathogenicity' named entity 'piglet' named entity 'PEDV' named entity 'codons' named entity 'vaccine' named entity 'fetal bovine serum' named entity 'PEDV' named entity 'deletions' named entity 'passaged' named entity 'United States' named entity 'antisera' named entity 'titer' named entity 'Alphacoronavirus' named entity 'genomes' named entity 'MAb' named entity 'small intestines' named entity 'Nidovirales' named entity 'microtiter plates'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software