About: Triptolide-induced apoptosis in non-small cell lung cancer via a novel miR204-5p/Caveolin-1/Akt-mediated pathway

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About: Triptolide-induced apoptosis in non-small cell lung cancer via a novel miR204-5p/Caveolin-1/Akt-mediated pathway Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Triptolide-induced apoptosis in non-small cell lung cancer via a novel miR204-5p/Caveolin-1/Akt-mediated pathway
Creator	Kumar, Ajay Ryan, John Burki, Sarah D'cunha, Jonathan Noda, Kentaro Philips, Brian
topic	covid:f95cb2f870e0168fb9010e33474e0b3bf9109000#this
Source	Medline; PMC
abstract	Lung cancer is one of the most prevalent malignancies world-wide with non-small cell lung cancer (NSCLC) comprising nearly 80% of all cases. Unfortunately, many lung cancer patients are diagnosed at advanced stages of the disease with an associated poor prognosis. Recently, the Chinese herb root extract Triptolide/Minnelide (TL) has shown significant promise as a therapeutic agent for NSCLC treatment both in vitro and in vivo. The aim of this study was to investigate the underlying mechanism(s) of action regarding TL-induced cytotoxicity in NSCLC. We demonstrate that triptolide treatment of A549 and H460 NSCLC cells decreases Caveolin-1 (CAV-1) mRNA/protein expression, resulting in activation of the Akt/Bcl-2-mediated mitochondrial apoptosis pathway. CAV-1 down-regulation was triggered by Micro-RNA 204-5p (miR204-5p) up-regulation and could be significantly blocked by pre-treatment with both Sirt-1/Sirt-3 specific siRNA and SIRT-1/SIRT-3 enzyme inhibitors, EX-527 and nicotinamide. Overall, our results provide evidence for a novel mechanism by which TL exerts its cytotoxic effects on NSCLC via CAV-1 down-regulation. Furthermore, these findings demonstrate a pivotal role for TL induction of the Akt/Bax pathway in apoptosis of human lung cancer.
has issue date	2020-07-14(xsd:dateTime)
bibo:doi	10.18632/oncotarget.27672
bibo:pmid	32733649
has license	cc-by
sha1sum (hex)	f95cb2f870e0168fb9010e33474e0b3bf9109000
schema:url	https://doi.org/10.18632/oncotarget.27672
resource representing a document's title	Triptolide-induced apoptosis in non-small cell lung cancer via a novel miR204-5p/Caveolin-1/Akt-mediated pathway
has PubMed Central identifier	PMC7367654
has PubMed identifier	32733649
schema:publication	Oncotarget
resource representing a document's body	covid:f95cb2f870e0168fb9010e33474e0b3bf9109000#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/f95cb2f870e0168fb9010e33474e0b3bf9109000
is schema:about of	named entity 'Bcl-2' named entity 'NSCLC' named entity 'non-small cell lung cancer' named entity 'Caveolin-1' named entity 'Sirt' named entity 'Lung cancer' named entity 'Sirt' named entity 'malignancies' named entity 'NSCLC' named entity 'protein' named entity 'mRNA' named entity 'Akt' named entity 'up-regulation' named entity 'Akt' named entity 'apoptosis' named entity 'phosphorylation' named entity 'protein expression' named entity 'signaling protein' named entity 'Sigma-Aldrich' named entity 'reverse transcription PCR' named entity 'Carlsbad, CA' named entity 'mRNA' named entity 'NSCLC' named entity 'CO2' named entity 'Triptolide' named entity 'Poly (ADP-ribose) polymerase' named entity 'NSCLC' named entity 'caveolin-1' named entity 't-test' named entity 'A549' named entity 'proapoptotic' named entity 'non-coding' named entity 'cancer cells' named entity 'NCI-H460' named entity 'up-regulation' named entity 'NSCLC' named entity 'cell survival' named entity 'primary antibodies' named entity 'cell lines' named entity 'University of Pittsburgh' named entity 'lung cancer' named entity 'Carbazole' named entity 'cDNA' named entity 'glycerol' named entity 'Snord44' named entity 'protein expression' named entity 'cytotoxicity' named entity 'Real-time PCR' named entity 'SYBR Green' named entity 'MicroRNAs' named entity 'A549' named entity 'cytotoxic effects' named entity 'A549' named entity 'mitochondrial function' named entity 'A549' named entity 'xenograft' named entity 'apoptosis' named entity 'anti-inflammatory' named entity 'cultured cells' named entity 'protein' named entity 'proliferation, development' named entity 'NSCLC' named entity 'JAK1/STAT3' named entity 'SYBR Green' named entity 'NSCLC' named entity 'Ser473' named entity 'protein expression' named entity 'serum' named entity 'mRNA expression' named entity 'mRNA transcript' named entity 'mRNA' named entity 'A549' named entity 'down-regulation' named entity 'NCI-H460'

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