About: Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6

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About: Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6
Creator	Lamarre, Alain Daniel, Claude Talbot, Pierre
topic	covid:fa8c05000debca85cd90a78312909515be903659#this
Source	Elsevier; Medline; PMC
abstract	Abstract Infection of cell monolayers by murine coronavirus A59 at pH 6 rather than 7 yielded a ten-fold increase in the infectious titer and a remarkable enhancement of the reactivities of monoclonal and polyclonal antibodies against the spike glycoprotein in immunoblotting, immuno-precipitation and enzyme-linked immunosorbent assays. These observations are very useful for detecting antibodies against the S glycoprotein of coronaviruses and enhancing infectious titers.
has issue date	1994-12-31(xsd:dateTime)
bibo:doi	10.1016/0166-0934(94)90180-5
bibo:pmid	7714047
has license	els-covid
sha1sum (hex)	fa8c05000debca85cd90a78312909515be903659
schema:url	https://doi.org/10.1016/0166-0934%2894%2990180-5
resource representing a document's title	Increased viral titers and enhanced reactivity of antibodies to the spike glycoprotein of murine coronavirus produced by infection at pH 6
has PubMed Central identifier	PMC7119785
has PubMed identifier	7714047
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:fa8c05000debca85cd90a78312909515be903659#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/fa8c05000debca85cd90a78312909515be903659
is schema:about of	named entity 'immunoblotting' named entity 'murine coronavirus' named entity 'INCREASED' named entity 'OBSERVATIONS' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAYS' named entity 'VIRAL' named entity 'IMMUNOBLOTTING' named entity 'ANTIBODIES' named entity 'MONOCLONAL' named entity 'MONOLAYERS' named entity 'INFECTIOUS' named entity 'THESE' named entity 'DETECTING' named entity 'RATHER' named entity 'ENHANCED' named entity 'REACTIVITY' named entity 'MURINE CORONAVIRUS' named entity 'FOLD INCREASE' named entity 'INFECTION' named entity 'ENHANCING' named entity 'USEFUL' named entity 'ANTIBODIES' named entity 'MURINE CORONAVIRUS' named entity 'TITER' named entity 'A59' named entity 'POLYCLONAL ANTIBODIES' named entity 'IMMUNOPRECIPITATION' named entity 'CORONAVIRUSES' named entity 'INFECTION' named entity 'ENHANCEMENT' named entity 'CELL' named entity 'virus' named entity 'A59' named entity 'cell fusion' named entity 'supernatants' named entity 'syncytia' named entity 'C-terminal' named entity 'serum' named entity 'antisera' named entity 'ascites fluid' named entity 'gastrointestinal' named entity 'cell line' named entity 'RNA viruses' named entity 'culture medium' named entity 'low affinity' named entity 'hybridoma' named entity 'Western blot' named entity 'antibodies' named entity 'multinucleated' named entity 'cell cultures' named entity 'antiserum' named entity 'antibodies' named entity 'hydrogen peroxide' named entity 'immunogenicity' named entity 'infection' named entity 'nitrocellulose' named entity 'nucleoprotein' named entity 'structural proteins' named entity 'infection' named entity 'produced' named entity 'coronaviruses' named entity 'titer' named entity 'enzyme-linked immunosorbent assays' named entity 'incubation' named entity 'viral titers' named entity 'immunoblotting' named entity 'incubation' named entity 'E17' named entity 'Scripps Clinic' named entity 'murine coronavirus' named entity 'A59' named entity 'antibodies' named entity 'N-terminal' named entity 'cytopathic effects' named entity 'Research Institute' named entity 'conformation' named entity 'protein'

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