About: Use of synthetic oligonucleotide probes to detect rhinovirus RNA

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Use of synthetic oligonucleotide probes to detect rhinovirus RNA
Creator	Bruce, Christine Tyrrell, D A1-Nakib, W Almond, J
Source	Medline; PMC
abstract	Current methods of detecting a human rhinovirus (HRV) infection are either based on isolation of virus in appropriate susceptible cell lines, which is time-consuming and requires considerable expertise, or are dependent on knowing the serotype. The extistence of over 100 immunologically distinct serotypes makes serotype specific assays, such as ELISA, unsuitable for general diagnostic assays. In this study a general rhinovirus assay is described which utilises synthetic oligonucleotides as probes in a filter hybridization assay. The probes are designed to bind to short but highly conserved regions of the rhinovirus genome. Indeed, the probes successfully detected all 57 rhinovirus serotypes tested. Furthermore, the test was used to demonstrate rhinovirus infection in clinical samples from 57 volunteers, inoculated with HRV, collected on six consecutive days. Clinical samples were taken prior to inoculation and on days 2–7 after inoculation. The filter hybridization assay gave results comparable to virus culture on days 2 and 3 post-inoculation, but was more sensitive on subsequent days.
has issue date	1989-01-01(xsd:dateTime)
bibo:doi	10.1007/bf01311355
bibo:pmid	2546516
has license	no-cc
sha1sum (hex)	faa60bdd274de9ef50b5529637ee0c6d5105652c
schema:url	https://doi.org/10.1007/bf01311355
resource representing a document's title	Use of synthetic oligonucleotide probes to detect rhinovirus RNA
has PubMed Central identifier	PMC7086780
has PubMed identifier	2546516
schema:publication	Arch Virol
resource representing a document's body	covid:faa60bdd274de9ef50b5529637ee0c6d5105652c#body_text
is schema:about of	named entity 'susceptible' named entity 'serotype' named entity 'serotypes' named entity 'rhinovirus' named entity 'SYNTHETIC' named entity 'VIRUS' named entity 'RHINOVIRUS INFECTION' named entity 'ELISA' named entity 'DESCRIBED' named entity 'DETECTED' named entity 'FILTER' named entity '2-7' named entity 'DISTINCT' named entity 'SENSITIVE' named entity 'USED' named entity 'HIGHLY' named entity 'GENOME' named entity 'DIAGNOSTIC' named entity 'VOLUNTEERS' covid:arg/faa60bdd274de9ef50b5529637ee0c6d5105652c named entity 'study' named entity 'tested' named entity 'nucleotide sequence' named entity 'HRV' named entity '0.01' named entity 'precipitate' named entity 'oligonucleotides' named entity 'proteinase' named entity 'hybridization' named entity 'intranasally' named entity 'neutralizing antibody' named entity 'infection' named entity 'rhinovirus' named entity 'mCi' named entity 'immunologically' named entity 'filter' named entity 'virus' named entity 'genome' named entity 'hybridization assay' named entity 'Viruses' named entity 'reoviruses' named entity 'infection' named entity 'reagent' named entity 'antibody' named entity 'homology' named entity 'RNA' named entity 'chloroform' named entity 'supernatant' named entity 'enteroviruses' named entity 'ATP' named entity 'chemotherapy' named entity 'formaldehyde' named entity 'virus' named entity 'rhinovirus' named entity 'rhinovirus' named entity 'virus' named entity 'chloroform' named entity 'sensitive' named entity 'hybridization' named entity 'probes' named entity 'probes' named entity 'conserved regions' named entity 'genome' named entity 'rhinovirus' named entity 'clinical samples' named entity 'human rhinovirus' named entity 'inoculation' named entity 'rhinovirus' named entity 'serotypes' named entity 'diagnostic assays' named entity 'inoculation' named entity 'serotype' named entity 'cell lines' named entity 'rhinovirus' named entity 'X-ray film' named entity 'biotinylated' named entity 'HRV' named entity 'viral RNA'

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