About: Identification and Characterization of a Novel Non-Structural Protein of Bluetongue Virus

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About: Identification and Characterization of a Novel Non-Structural Protein of Bluetongue Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Identification and Characterization of a Novel Non-Structural Protein of Bluetongue Virus
Creator	Allan, K Caporale, M Franzoni, G Golder, M Ratinier, M
source	PMC
abstract	Bluetongue virus (BTV) is the causative agent of a major disease of livestock (bluetongue). For over two decades, it has been widely accepted that the 10 segments of the dsRNA genome of BTV encode for 7 structural and 3 non-structural proteins. The non-structural proteins (NS1, NS2, NS3/NS3a) play different key roles during the viral replication cycle. In this study we show that BTV expresses a fourth non-structural protein (that we designated NS4) encoded by an open reading frame in segment 9 overlapping the open reading frame encoding VP6. NS4 is 77–79 amino acid residues in length and highly conserved among several BTV serotypes/strains. NS4 was expressed early post-infection and localized in the nucleoli of BTV infected cells. By reverse genetics, we showed that NS4 is dispensable for BTV replication in vitro, both in mammalian and insect cells, and does not affect viral virulence in murine models of bluetongue infection. Interestingly, NS4 conferred a replication advantage to BTV-8, but not to BTV-1, in cells in an interferon (IFN)-induced antiviral state. However, the BTV-1 NS4 conferred a replication advantage both to a BTV-8 reassortant containing the entire segment 9 of BTV-1 and to a BTV-8 mutant with the NS4 identical to the homologous BTV-1 protein. Collectively, this study suggests that NS4 plays an important role in virus-host interaction and is one of the mechanisms played, at least by BTV-8, to counteract the antiviral response of the host. In addition, the distinct nucleolar localization of NS4, being expressed by a virus that replicates exclusively in the cytoplasm, offers new avenues to investigate the multiple roles played by the nucleolus in the biology of the cell.
has issue date	2011-12-29(xsd:dateTime)
bibo:doi	10.1371/journal.ppat.1002477
bibo:pmid	22241985
has license	cc-by
sha1sum (hex)	fc9a6a00498ee4dfeaf7c201c3341aca6ad21409
schema:url	https://doi.org/10.1371/journal.ppat.1002477
resource representing a document's title	Identification and Characterization of a Novel Non-Structural Protein of Bluetongue Virus
has PubMed Central identifier	PMC3248566
has PubMed identifier	22241985
schema:publication	PLoS Pathog
resource representing a document's body	covid:fc9a6a00498ee4dfeaf7c201c3341aca6ad21409#body_text
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