About: Immune response development after vaccination of 1-day-old naïve pigs with a Porcine Reproductive and Respiratory Syndrome 1-based modified live virus vaccine

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About: Immune response development after vaccination of 1-day-old naïve pigs with a Porcine Reproductive and Respiratory Syndrome 1-based modified live virus vaccine Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Immune response development after vaccination of 1-day-old naïve pigs with a Porcine Reproductive and Respiratory Syndrome 1-based modified live virus vaccine
Creator	Mateu, Enric Balasch, Monica Díaz, Ivan Fort, Maria Calvert, Jay Taylor, Lucas
Source	Medline; PMC
abstract	BACKGROUND: The development of the innate and adaptive immune responses to Porcine reproductive and respiratory syndrome virus (PRRSV) after vaccination of 1 day-old pigs with a PRRSV-1 based modified live virus (MLV) vaccine by intramuscular (IM) and intranasal (IN) routes was characterised, before and after challenge with a heterologous PRRSV-1 isolate at 18 weeks post-vaccination. Twenty-five PRRSV-seronegative piglets were used. At 1 day of age, pigs were administered with a single dose of vaccine via the IM (n = 10) or the IN route (n = 10). Control group (n = 5) received saline solution. After vaccination, pigs were bled at days 3, 7, 28, 56, 83, 113 and 125. Levels of cytokines IL-10, IL-8, IFN-α (measured by ELISA tests of serum), TNF-α and IFN-γ (measured by ELISA and ELISPOT, respectively, from stimulated peripheral blood mononuclear cells), and serum neutralising antibodies (NA) to the vaccine strain, were measured. RESULTS: The induction of IL-10 was rare, indicating that IL-10 mediated immunomodulation/immune dysfunction was not a feature of this vaccine or of the challenge virus. IL-8 was detected in only two pigs following vaccination, but in the majority of pigs after challenge, indicating that their ability to produce an innate immune response was not impaired. TNF-α was not detected in any vaccinated pigs until day 83. After challenge, only a minority of pigs produced TNF-α. IFN-α was detected in all vaccinated pigs following vaccination, indicating the potential for development of an effective Th1 adaptive immune response. IFN-γ-secreting cells were detected in all vaccinated pigs after vaccination. NA to the vaccine strain were first detected at day 56 in pigs vaccinated by both routes, and remained at similar levels until challenge. After challenge, a boost in NA was observed. The efficacy of the vaccine was demonstrated by reduction of viraemia and nasal shedding after challenge. CONCLUSIONS: The administration of a PRRSV-1 based MLV vaccine to 1 day-old piglets was able to induce an immune response characterised by: (1) undetectable or low levels of IL-10, IL-8 and TNF-α, (2) an increase in IFN-α expression within the first seven days, (3) a gradual increase in the number of antigen-specific IFN-γ-secreting cells, and (4) induction of detectable NA. After challenge with a heterologous strain, there was a rapid boost in NA titres, indicating a priming effect of the vaccine.
has issue date	2019-02-02(xsd:dateTime)
bibo:doi	10.1186/s40813-018-0112-7
bibo:pmid	30761215
has license	cc-by
sha1sum (hex)	fe14106f9f3d2b3b6d8ea619cfcc4363148dc84b
schema:url	https://doi.org/10.1186/s40813-018-0112-7
resource representing a document's title	Immune response development after vaccination of 1-day-old naïve pigs with a Porcine Reproductive and Respiratory Syndrome 1-based modified live virus vaccine
has PubMed Central identifier	PMC6359793
has PubMed identifier	30761215
schema:publication	Porcine Health Manag
resource representing a document's body	covid:fe14106f9f3d2b3b6d8ea619cfcc4363148dc84b#body_text
is schema:about of	named entity 'antibodies' named entity 'stimulated' named entity 'route' named entity 'intramuscular' named entity 'virus' named entity 'DAY-OLD' named entity 'SINGLE' named entity 'USED' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS' named entity 'ROUTE' named entity 'TESTS' named entity 'DOSE' named entity 'RECEIVED' named entity 'IL-8' named entity 'IFN' named entity 'VACCINE' named entity 'LEVELS' named entity '125' named entity 'VACCINATION' named entity 'BACKGROUND' named entity 'BLED' named entity '1 DAY' named entity 'ELISPOT' named entity 'SALINE SOLUTION' named entity 'VACCINE STRAIN' named entity 'ADMINISTERED' named entity 'CHALLENGE' named entity 'AGE' named entity 'DAYS' named entity 'POST' named entity 'MODIFIED' named entity 'PIGS' named entity 'INTRANASAL' named entity '18 WEEKS' named entity 'MODIFIED' named entity 'ISOLATE' named entity 'VIRUS' named entity 'ADAPTIVE IMMUNE RESPONSES' named entity 'ELISA' named entity 'CONTROL GROUP' named entity 'VIRUS VACCINE' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME' named entity 'VACCINATION' named entity 'BASED' named entity 'SERONEGATIVE' named entity '10%' named entity 'STIMULATED' named entity 'INTRAMUSCULAR' named entity 'BASED' named entity 'IL-10' named entity 'DAY-OLD' named entity 'DEVELOPMENT' named entity 'NEUTRALISING ANTIBODIES' named entity 'MEASURED' named entity 'TWENTY-FIVE' named entity 'ROUTES' named entity 'IMMUNE RESPONSE' named entity 'LIVE' named entity 'SERUM' named entity 'PIGS' named entity 'HETEROLOGOUS' named entity 'PERIPHERAL BLOOD MONONUCLEAR CELLS' named entity 'CYTOKINES' named entity 'LIVE' named entity 'DEVELOPMENT' covid:arg/fe14106f9f3d2b3b6d8ea619cfcc4363148dc84b named entity 'tests' named entity 'vaccine' named entity 'heterologous' named entity 'intranasal' named entity 'Syndrome' named entity 'modified live' named entity 'adaptive immune responses' named entity 'cytokines' named entity 'saline solution' named entity 'Control group'

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