About: A specific sequence in the genome of respiratory syncytial virus regulates the generation of copy-back defective viral genomes

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About: A specific sequence in the genome of respiratory syncytial virus regulates the generation of copy-back defective viral genomes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A specific sequence in the genome of respiratory syncytial virus regulates the generation of copy-back defective viral genomes
Creator	Agarwal, Divyansh Kim, Eun Bastien, Sé Felt, A Grant, Gregory Id, Louis Id, Yan Ló Pez, Carolina
source	PMC
abstract	Defective viral genomes of the copy-back type (cbDVGs) are the primary initiators of the antiviral immune response during infection with respiratory syncytial virus (RSV) both in vitro and in vivo. However, the mechanism governing cbDVG generation remains unknown, thereby limiting our ability to manipulate cbDVG content in order to modulate the host response to infection. Here we report a specific genomic signal that mediates the generation of a subset of RSV cbDVG species. Using a customized bioinformatics tool, we identified regions in the RSV genome frequently used to generate cbDVGs during infection. We then created a minigenome system to validate the function of one of these sequences and to determine if specific nucleotides were essential for cbDVG generation at that position. Further, we created a recombinant virus unable to produce a subset of cbDVGs due to mutations introduced in this sequence. The identified sequence was also found as a site for cbDVG generation during natural RSV infections, and common cbDVGs originated at this sequence were found among samples from various infected patients. These data demonstrate that sequences encoded in the viral genome determine the location of cbDVG formation and, therefore, the generation of cbDVGs is not a stochastic process. These findings open the possibility of genetically manipulating cbDVG formation to modulate infection outcome.
has issue date	2019-04-17(xsd:dateTime)
bibo:doi	10.1371/journal.ppat.1007707
bibo:pmid	30995283
has license	cc-by
sha1sum (hex)	fe53e5ca071be4ba98a8366548192fc00d35333b
schema:url	https://doi.org/10.1371/journal.ppat.1007707
resource representing a document's title	A specific sequence in the genome of respiratory syncytial virus regulates the generation of copy-back defective viral genomes
has PubMed Central identifier	PMC6504078
has PubMed identifier	30995283
schema:publication	PLoS Pathog
resource representing a document's body	covid:fe53e5ca071be4ba98a8366548192fc00d35333b#body_text
is schema:about of	named entity 'The' named entity 'infections' named entity 'infection' named entity 'RSV' named entity 'viral genomes' named entity 'ABILITY TO' named entity 'ANTIVIRAL IMMUNE RESPONSE' named entity 'DUE TO' named entity 'ESSENTIAL' covid:arg/fe53e5ca071be4ba98a8366548192fc00d35333b named entity 'SYSTEM' named entity 'IDENTIFIED' named entity 'ENCODED' named entity 'IN VITRO' named entity 'COPY' named entity 'RESPIRATORY SYNCYTIAL VIRUS' named entity 'RECOMBINANT VIRUS' named entity 'GENERATION' named entity 'DATA' named entity 'VIRAL' named entity 'UNKNOWN' named entity 'GENERATE' named entity 'MUTATIONS' named entity 'INFECTED' named entity 'FOUND' named entity 'LOCATION' named entity 'NUCLEOTIDES' named entity 'GENOME' named entity 'SPECIFIC' named entity 'TO PRODUCE' named entity 'GENOMIC' named entity 'PATIENTS' named entity 'GENOME' named entity 'GENOMES' named entity 'SPECIFIC' named entity 'VIRAL' named entity 'RESPIRATORY SYNCYTIAL VIRUS' named entity 'GENERATION' named entity 'RSV INFECTIONS' named entity 'GENOMES' named entity 'IN VIVO' named entity 'MEDIATES' named entity 'USING' named entity 'SEQUENCES' named entity 'MANIPULATE' named entity 'THESE' named entity 'HOST RESPONSE' named entity 'SIGNAL' named entity 'DETERMINE' named entity 'SPECIES' named entity 'FREQUENTLY' named entity 'FORMATION' named entity 'USED' named entity 'INTRODUCED' named entity 'MANIPULATING' named entity 'SUBSET' named entity 'MECHANISM' named entity 'SAMPLES' named entity 'ORDER' named entity 'COPY' named entity 'OUR' named entity 'VIRAL GENOME' named entity 'FUNCTION' named entity 'OUTCOME' named entity 'HERE' named entity 'ONE OF' named entity 'NATURAL' named entity 'CUSTOMIZED' named entity 'STOCHASTIC PROCESS' named entity 'PRIMARY' named entity 'CONTENT' named entity 'SITE' named entity 'FINDINGS' named entity 'POSSIBILITY OF'

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