About: Molecular Mapping of the RNA Cap 2′-O-Methyltransferase Activation Interface between Severe Acute Respiratory Syndrome Coronavirus nsp10 and nsp16

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About: Molecular Mapping of the RNA Cap 2′-O-Methyltransferase Activation Interface between Severe Acute Respiratory Syndrome Coronavirus nsp10 and nsp16 Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecular Mapping of the RNA Cap 2′-O-Methyltransferase Activation Interface between Severe Acute Respiratory Syndrome Coronavirus nsp10 and nsp16
Creator	Canard, Bruno Decroly, Etienne Debarnot, Claire Guillemot, Jean-Claude Lugari, Adrien Morelli, Xavier Betzi, Stephane Bonnaud, Emmanuel Borg, Jean-Paul Lé Cine ¶ʈ, Patrick
Source	Medline; PMC
abstract	Several protein-protein interactions within the SARS-CoV proteome have been identified, one of them being between non-structural proteins nsp10 and nsp16. In this work, we have mapped key residues on the nsp10 surface involved in this interaction. Alanine-scanning mutagenesis, bioinformatics, and molecular modeling were used to identify several “hot spots,” such as Val(42), Met(44), Ala(71), Lys(93), Gly(94), and Tyr(96), forming a continuous protein-protein surface of about 830 Å(2), bearing very conserved amino acids among coronaviruses. Because nsp16 carries RNA cap 2′-O-methyltransferase (2′O-MTase) activity only in the presence of its interacting partner nsp10 (Bouvet, M., Debarnot, C., Imbert, I., Selisko, B., Snijder, E. J., Canard, B., and Decroly, E. (2010) PLoS Pathog. 6, e1000863), functional consequences of mutations on this surface were evaluated biochemically. Most changes that disrupted the nsp10-nsp16 interaction without structural perturbations were shown to abrogate stimulation of nsp16 RNA cap 2′O-MTase activity. More strikingly, the Y96A mutation abrogates stimulation of nsp16 2′O-MTase activity, whereas Y96F overstimulates it. Thus, the nsp10-nsp16 interface may represent an attractive target for antivirals against human and animal pathogenic coronaviruses.
has issue date	2010-08-10(xsd:dateTime)
bibo:doi	10.1074/jbc.m110.120014
bibo:pmid	20699222
has license	bronze-oa
sha1sum (hex)	fe95d1df08d5ebe24270d828705cfc449ab119e3
schema:url	https://doi.org/10.1074/jbc.m110.120014
resource representing a document's title	Molecular Mapping of the RNA Cap 2′-O-Methyltransferase Activation Interface between Severe Acute Respiratory Syndrome Coronavirus nsp10 and nsp16
has PubMed Central identifier	PMC2963367
has PubMed identifier	20699222
schema:publication	Journal of Biological Chemistry
resource representing a document's body	covid:fe95d1df08d5ebe24270d828705cfc449ab119e3#body_text
is schema:about of	named entity 'CAP 2' named entity 'O-METHYLTRANSFERASE' named entity 'molecular modeling' named entity 'conserved' named entity 'represent' named entity 'key' named entity 'interacting' named entity 'Met' named entity 'SARS-CoV' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'HAVE' named entity 'NONSTRUCTURAL PROTEINS' named entity 'SARS-COV' named entity 'TO IDENTIFY' named entity 'IDENTIFIED' named entity 'ABOUT' named entity 'PROTEIN-PROTEIN INTERACTIONS' named entity 'GLY' named entity 'ITS' named entity 'PATHOGENIC' named entity 'PROTEOME' named entity 'EVALUATED' named entity 'CANARD' named entity 'CAP' named entity 'CONSEQUENCES' named entity 'CORONAVIRUSES' named entity 'CARRIES' named entity 'MAPPED' named entity 'PROTEIN SURFACE' named entity 'SCANNING' named entity 'MUTATION' named entity 'INTERFACE' named entity 'ACTIVATION' named entity 'CHANGES' named entity 'LYS' named entity 'MTASE' named entity 'SPOTS' named entity 'O-METHYLTRANSFERASE' named entity 'VAL' named entity 'INVOLVED' named entity 'STRUCTURAL' named entity 'CAP 2' named entity 'ATTRACTIVE' named entity 'ANTIVIRALS' named entity 'FORMING' named entity 'TARGET' named entity 'WORK' named entity 'AMINO ACIDS' named entity 'FUNCTIONAL' named entity 'TYR' named entity 'REPRESENT' named entity 'INTERACTION' named entity 'ALANINE' named entity 'MOLECULAR MAPPING' named entity 'RNA' named entity 'STIMULATION' named entity 'BIOINFORMATICS' named entity 'BEING' named entity 'INTERFACE' named entity 'ONE OF' named entity 'PARTNER' named entity 'USED' named entity 'MUTAGENESIS' named entity 'PROTEIN-PROTEIN' named entity 'ACTIVITY' named entity '830' named entity 'SURFACE' named entity 'BEARING' named entity 'ANIMAL' named entity 'DISRUPTED' named entity 'MOLECULAR MODELING' named entity 'ALA'

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