About: Frequency of BCoV detection by a semi-nested PCR assay in faeces of calves from Brazilian cattle herds

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About: Frequency of BCoV detection by a semi-nested PCR assay in faeces of calves from Brazilian cattle herds Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Frequency of BCoV detection by a semi-nested PCR assay in faeces of calves from Brazilian cattle herds
Creator	Takiuchi, Elisabete Alfieri, Alice Alfieri, Amauri Amude, Alexandre Barry, Aline Stipp, Danilo
Source	Medline; PMC
abstract	Bovine coronavirus (BCoV) is one of the main causes of neonatal calf diarrhoea. Several diagnostic assays have been employed to detect the presence of the virus in stool samples from calves. Despite this, the frequency of BCoV infection among Brazilian and even South American cattle herds has yet to be well characterised. This study describes the occurrence of BCoV infection among calves from dairy and beef herds in four Brazilian states. A total of 282 stool samples from 1 to 60-day-old calves were evaluated for the presence of BCoV by a semi-nested (SN) PCR assay. The animals were from herds (n = 23) located in three geographical regions in Brazil (south, southeast, and center-west). The specific BCoV amplicon was detected in 15.6% (44/282) of the faecal specimens examined, of which 95.4% (42/44) were from diarrhoeic and 4.6% (2/44) from asymptomatic calves. The specificity of the SN-PCR amplicons was evaluated by restriction fragment length polymorphism (RFLP) analysis. The results show that the BCoV is widespread, mainly among calves from 16 to 30-days-old (p = 0.0023), and verify the association between BCoV infection and clinical signs of diarrhoea (p = 0.005). These findings emphasise the importance of this virus in enteric infections of Brazilian cattle herds.
has issue date	2009-04-16(xsd:dateTime)
bibo:doi	10.1007/s11250-009-9347-2
bibo:pmid	19370396
has license	no-cc
sha1sum (hex)	feb29b8c51f4a521c4f373b83a37aee6c1a7e2a6
schema:url	https://doi.org/10.1007/s11250-009-9347-2
resource representing a document's title	Frequency of BCoV detection by a semi-nested PCR assay in faeces of calves from Brazilian cattle herds
has PubMed Central identifier	PMC7089217
has PubMed identifier	19370396
schema:publication	Trop Anim Health Prod
resource representing a document's body	covid:feb29b8c51f4a521c4f373b83a37aee6c1a7e2a6#body_text
is schema:about of	named entity 'dairy' named entity 'diarrhoea' named entity 'cattle' named entity 'detected' named entity 'Brazilian' named entity 'neonatal' named entity 'faecal' named entity 'infection' named entity 'polymorphism' named entity 'PCR' named entity 'FAECES' named entity 'ANIMALS' named entity 'SOUTH AMERICAN' named entity 'NEONATAL CALF DIARRHOEA' named entity 'TO DETECT' named entity 'DIAGNOSTIC' named entity 'CATTLE' named entity 'PCR' named entity 'WEST' named entity 'DETECTION' named entity 'EVALUATED' named entity 'BRAZIL' named entity 'OCCURRENCE' named entity 'VIRUS' named entity 'FREQUENCY' named entity 'SOUTHEAST' named entity 'RESTRICTION FRAGMENT LENGTH POLYMORPHISM ' named entity 'NESTED' named entity 'INFECTION' named entity 'LOCATED IN' named entity 'SAMPLES' named entity 'BEEF' named entity 'DAIRY' named entity 'WIDESPREAD' named entity 'BRAZILIAN' named entity 'NESTED PCR ' named entity 'CALVES' named entity 'PCR ASSAY' named entity '282' named entity 'STATES' named entity 'PRESENCE OF' named entity 'FREQUENCY' named entity 'CATTLE' named entity 'ANALYSIS' named entity 'FAECAL' named entity 'INFECTIONS' named entity 'ENTERIC' named entity 'ONE OF' named entity 'VERIFY' named entity 'HAVE' named entity 'RESULTS' named entity 'TOTAL' named entity 'EXAMINED' named entity 'OLD' named entity 'EMPLOYED' named entity 'PCR ASSAY' named entity 'BRAZILIAN' named entity 'MAIN' named entity '28P' named entity 'CAUSES' named entity 'DAYS' named entity 'AMPLICON' named entity 'FINDINGS' named entity 'STOOL' named entity 'STUDY' named entity 'SPECIFICITY' named entity 'GEOGRAPHICAL REGIONS' named entity 'DIARRHOEA' named entity 'THESE' named entity 'SPECIMENS' named entity 'CALVES' named entity 'ASSOCIATION' named entity 'DETECTED' named entity 'DAY-OLD' named entity 'CENTER' named entity '4.6'

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