About: Characterization of monoclonal antibodies against feline coronavirus accessory protein 7b

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About: Characterization of monoclonal antibodies against feline coronavirus accessory protein 7b Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characterization of monoclonal antibodies against feline coronavirus accessory protein 7b
Creator	Tekes, Gergely Thiel, Heinz-Jürgen Ziebuhr, John Lamp, Benjamin Lemmermeyer, Tanja Schneider, Rainer
topic	covid:ff95dcd75515b1bd9dbb66bc58f495c42159676e#this
Source	Elsevier; Medline; PMC
abstract	Feline coronaviruses (FCoVs) encode five accessory proteins termed 3a, 3b, 3c, 7a and 7b of unknown function. These proteins are dispensable for viral replication in vitro but are supposed to play a role in virulence. In the current study, we produced and characterized 7b-specific monoclonal antibodies (mAbs). A recombinant form of the 7b protein was expressed as a fusion protein in Escherichia coli, purified by immobilized metal affinity chromatography and used as immunogen. Two hybridoma lines, 5B6 and 14D8, were isolated that expressed mAbs that recognized 7b proteins of both FCoV serotypes. Using an extensive set of N- and C-terminally truncated 7b proteins expressed in E. coli and a synthetic peptide, the binding sites of mAbs 5B6 and 14D8 were mapped to an 18-residue region that comprises the only potential N-glycosylation site of the FCoV 7b protein. The two mAbs were suitable to detect a 24-kDa protein, which represents the nonglycosylated form of 7b in FCoV-infected cells. We speculate that glycosylation of 7b is part of the viral evasion strategy to prevent an immune response against this antigenic site.
has issue date	2016-02-29(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2015.12.009
bibo:pmid	26854339
has license	no-cc
sha1sum (hex)	ff95dcd75515b1bd9dbb66bc58f495c42159676e
schema:url	https://doi.org/10.1016/j.vetmic.2015.12.009
resource representing a document's title	Characterization of monoclonal antibodies against feline coronavirus accessory protein 7b
has PubMed Central identifier	PMC7117465
has PubMed identifier	26854339
schema:publication	Vet Microbiol
resource representing a document's body	covid:ff95dcd75515b1bd9dbb66bc58f495c42159676e#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/ff95dcd75515b1bd9dbb66bc58f495c42159676e
is schema:about of	named entity 'proteins' named entity 'mapped' named entity 'antigenic' named entity 'site' named entity 'fusion protein' named entity 'monoclonal antibodies' named entity 'feline coronavirus' named entity 'IN VITRO' named entity 'TRUNCATED' named entity 'SITE OF' named entity 'LINES' named entity 'THESE' named entity 'PLAY' named entity 'ENCODE' named entity 'USING' named entity 'FELINE' named entity 'EXTENSIVE' named entity 'IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY' named entity 'TO DETECT' named entity 'FELINE CORONAVIRUS' named entity 'ACCESSORY PROTEIN' covid:arg/ff95dcd75515b1bd9dbb66bc58f495c42159676e named entity 'MAPPED' named entity 'GLYCOSYLATION' named entity 'MONOCLONAL ANTIBODIES' named entity 'CHARACTERIZATION' named entity 'PROTEIN ' named entity 'ESCHERICHIA COLI' named entity 'BUT' named entity 'TO PREVENT' named entity 'PURIFIED' named entity 'N-GLYCOSYLATION SITE' named entity 'FUSION PROTEIN' named entity 'ACCESSORY' named entity 'PROTEIN ' named entity 'FUNCTION' named entity 'VIRAL REPLICATION' named entity 'VIRAL' named entity 'ISOLATED' named entity 'BINDING SITES' named entity 'REGION' named entity 'MONOCLONAL ANTIBODIES' named entity 'USED' named entity 'KDA' named entity 'RECOMBINANT' named entity 'IMMUNOGEN' named entity 'SITE' named entity 'EXPRESSED' named entity 'E. COLI' named entity 'CELLS' named entity 'STRATEGY' named entity 'RESIDUE' named entity 'SPECIFIC' named entity 'SYNTHETIC PEPTIDE' named entity 'UNKNOWN' named entity 'CHARACTERIZED' named entity 'HYBRIDOMA' named entity 'SEROTYPES' named entity 'SET' named entity 'INFECTED' named entity 'CURRENT' named entity 'CORONAVIRUSES' named entity 'IMMUNE RESPONSE' named entity 'POTENTIAL' named entity 'PART OF' named entity 'PROTEIN' named entity 'VIRULENCE' named entity 'PROTEINS' named entity 'FORM' named entity 'ROLE' named entity 'SUITABLE' named entity 'STUDY' named entity 'isolated' named entity 'Two'

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