About: chromatofocusing column

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An Entity of Type : owl:Class, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Class
label	chromatofocusing column
subClassOf	chromatography column
described by	proxy:entity/http/purl.obolibrary.org/obo/obi.owl
term editor	PERSON:Daniel Schober
definition	A chromatofocusing column is a chromatography column in which a resin is equilibrated at one pH and eluted at a second pH. The use of a weak ion-exchange resin causes a pH gradient to be formed at the solvent front owing to the buffering action of the resin. This pH gradient in turn leads to an ordering of proteins by isoelectric point. Molecules of charge sign opposite the resin bind; those of charge sign like the resin do not bind.
definition source	WEB:<http://www.bioprocessintl.com/default.asp?page=glossary&TopicID=1> http://msi-ontology.sourceforge.net/ontology/CHROM.owl#msi_01209
editor preferred term	chromatofocusing column
has curation status	metadata complete
is topic of	Ontology for Biomedical Investigations

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