About: enzyme-linked immunosorbent assay

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Attributes	Values
type	Class
label	enzyme-linked immunosorbent assay
subClassOf	analyte assay nodeID://b665184899 nodeID://b665209196 nodeID://b665807718 nodeID://b665832015 nodeID://b665864230 nodeID://b665888527 nodeID://b665912824 nodeID://b665937121
described by	https://covidontheweb.inria.fr:4443/about/id/entity/http/purl.obolibrary.org/obo/OBI_0001461 proxy:entity/http/purl.obolibrary.org/obo/obi.owl
term editor	IEDB
example of usage	1) Detection of IL-2 (analyte) in a cell supernatant (evaluant), using plate bound anti IL-2 antibodies, and a reporter enzyme-linked reporter antibody. 2) Measurement of IgG antibody (analyte) titer in a serum sample (evaluant) using plate bound antigen and a reporter anti-IgG antibody.
definition	An analyte assay where binding of an enzyme linked antibody to a material entity that is immobilized on solid support is detected utilizing a chemiluminescent reaction. Depending on the setup, the enzyme-linked antibody could be binding directly to the analyte, or it serves as a secondary antibody detecting binding of the primary antibody to the analyte.
definition source	IEDB
alternative label	ELISA
editor preferred term	enzyme-linked immunosorbent assay
has curation status	metadata complete
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