About: western blot assay   Goto Sponge  NotDistinct  Permalink

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AttributesValues
type
label
  • western blot assay
subClassOf
described by
term editor
  • Philippe Rocca-Serra
  • IEDB
example of usage
  • Running a cell lysate on an acrylamide gel in a western blot aparatus to separate the constituent proteins, followed by transfer of the proteins from the gel to a nitrocellulose membrane. Staining this membrane with specific antibodies to detect the presence of specific proteins of interest.
definition
  • An analyte assay that detects specific peptides in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with_ antibodies specific to the analyte molecules.
definition source
  • Philippe Rocca-Serra
  • IEDB
editor preferred term
  • western blot assay
has curation status
is first of
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