About: high performance liquid chromotography assay   Goto Sponge  NotDistinct  Permalink

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  • high performance liquid chromotography assay
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  • On-line coupled immunoaffinity chromatography-reversed-phase high-performance liquid chromatography (IAC-HPLC) with detection by quadrupole ion trap mass spectrometry using a particle beam interface has been developed for the determination of the steroids, dexamethasone and flumethasone. HEMA (polyhydroxyethylmethacrylate) was evaluated as a support material for the anti-dexamethasone antibodies used in IAC. Antibody cross-reactivity and non-specific binding have been investigated for the HEMA bound anti-dexamethasone IAC column. The on-line IAC-HPLC-MS determination of dexamethasone and flumethasone in post-administration equine urine samples showed precisions (R.S.D.) of 8.0 and 7.1%, respectively, with limits of detection in the range 3-4 ng/ml.
definition
  • An analytical chromatography assay that utilizes a high performance liquid chromatography instrument for separation of compounts in a solution.
definition source
  • PMID:9491555
alternative label
  • HPLC
editor preferred term
  • high performance liquid chromotography assay
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