About: precision nuclear run-on sequencing assay   Goto Sponge  NotDistinct  Permalink

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type
label
  • precision nuclear run-on sequencing assay
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described by
term tracker item
  • https://github.com/obi-ontology/obi/issues/876
term editor
  • Rebecca Jackson
definition
  • An RNA-seq assay that maps the genome-wide distribution of transcriptionally-engaged Pol II at base-pair resolution by using biotin-labeled ribonucleotide triphosphate analogs (biotin-NTP) for nuclear run-on reactions, allowing the efficient affinity purification of nascent RNAs for high throughput sequencing from their 3' ends.
  • An RNA-seq assay that maps the genome-wide distribution of transcriptionally-engaged Pol II at base-pair resolution by using biotin-labeled ribonucleotide triphosphate analogs (biotin-NTP) for nuclear run-on reactions, allowing the efficient affinity purification of nascent RNAs for high throughput sequencing from their 3' ends.
definition source
  • PMID:23430654
alternative label
  • PRO-Seq
  • precision nuclear run-on and sequencing assay
editor preferred term
  • precision nuclear run-on sequencing assay
has curation status
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