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Prefix	Namespace IRI
n4	http://localhost:8890/about/id/entity/http/purl.obolibrary.org/obo/obi.
n5	http://purl.obolibrary.org/obo/
wdrs	http://www.w3.org/2007/05/powder-s#
rdfs	http://www.w3.org/2000/01/rdf-schema#
rdf	http://www.w3.org/1999/02/22-rdf-syntax-ns#
owl	http://www.w3.org/2002/07/owl#
xsdh	http://www.w3.org/2001/XMLSchema#

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rdfs:label: Solexa sequencing assay
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n5:IAO_0000117: Philippe Rocca-Serra
n5:IAO_0000112: PMID: 18987734
n5:IAO_0000115: A DNA sequencing by synthesis assay that identifies sequence information using DNA polymerase and reversible terminator, requiring immobilization of genomic DNA fragments onto a surface and a specific clonal amplification step known as bridge PCR. Reliance on a reversible terminator allows cycles of DNA chain extension by DNA polymerase and imaging without the need of electrophoretic separation of newly synthesized DNA fragment as with Sanger sequencing. A DNA sequencing by synthesis assay that identifies sequence information using DNA polymerase and reversible terminator, requiring immobilization of genomic DNA fragments onto a surface and a specific clonal amplification step known as bridge PCR. Reliance on a reversible terminator allows cycles of DNA chain extension by DNA polymerase and imaging without the need of electrophoretic separation of newly synthesized DNA fragment as with Sanger sequencing.
n5:IAO_0000119: Solexa web site (SS_DNAsequencing.pdf document available on july 2009)
n5:IAO_0000118: reversible terminator sequencing
n5:IAO_0000111: Solexa sequencing assay
n5:IAO_0000114: n5:IAO_0000120

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