About: Inhibition of Hepatitis C Virus Infection in Cell Culture by Small Interfering RNAs

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About: Inhibition of Hepatitis C Virus Infection in Cell Culture by Small Interfering RNAs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Inhibition of Hepatitis C Virus Infection in Cell Culture by Small Interfering RNAs
Creator	Wychowski, Czeslaw Benureau, Yann Chevalier, Christophe Colbère-Garapin, Florence Delgrange, David Fléchet, Dorian Martin, Annette Saulnier, Aure
source	Elsevier; Medline; PMC
abstract	Hepatitis C virus (HCV) infection is a major cause of chronic liver disease and hepatocellular carcinoma, yet fully efficacious treatments are missing. In this study, we investigated RNA interference (RNAi), a specific gene silencing process mediated by small interfering RNA (siRNA) duplexes, as an antiviral strategy against HCV. Synthetic siRNAs were designed to target conserved sequences of the HCV 5′ nontranslated region (NTR) located in a functional, stem–loop structured domain of the HCV internal ribosome entry site (IRES), which is crucial for initiation of polyprotein translation. Several siRNAs dramatically reduced or even abrogated the replication of selectable subgenomic HCV replicons upon cotransfection of human hepatoma cells with viral target and siRNAs, or upon transfection of cells supporting autonomous replication of HCV replicon with siRNAs. Importantly, three siRNAs also proved capable of strongly inhibiting virus production in cell culture. One siRNA, targeting a sequence that is highly conserved across all genotypes and forms a critical pseudoknot structure involved in translation, was identified as the most promising therapeutic candidate. These results indicate that the HCV life cycle can be efficiently blocked by using properly-designed siRNAs that target functionally important, highly conserved sequences of the HCV IRES. This finding offers a novel approach towards developing IRES-based antiviral treatment for chronic HCV infections.
has issue date	2016-12-14(xsd:dateTime)
bibo:doi	10.1038/sj.mt.6300186
bibo:pmid	17505476
has license	no-cc
sha1sum (hex)	124d50f8e6f06f11cf9cf6349558d601790cdc34
schema:url	https://doi.org/10.1038/sj.mt.6300186
resource representing a document's title	Inhibition of Hepatitis C Virus Infection in Cell Culture by Small Interfering RNAs
has PubMed Central identifier	PMC7106008
has PubMed identifier	17505476
schema:publication	Mol Ther
resource representing a document's body	covid:124d50f8e6f06f11cf9cf6349558d601790cdc34#body_text
is schema:about of	named entity 'HCV' named entity 'This' named entity 'supporting' named entity 'target' named entity 'structured' named entity 'RNAs' covid:arg/124d50f8e6f06f11cf9cf6349558d601790cdc34 named entity 'RNAi' named entity 'translation' named entity 'chronic liver disease' named entity 'target' named entity 'cell culture' named entity 'One' named entity 'subgenomic' named entity 'siRNA' named entity 'siRNAs' named entity 'highly conserved' named entity 'HCV' named entity 'gene silencing' named entity 'chronic liver disease' named entity 'siRNAs' named entity 'siRNA' named entity 'internal ribosome entry site' named entity 'HCV' named entity 'small interfering RNA' named entity 'antiviral' named entity 'RNAi' named entity 'HCV IRES' named entity 'polyprotein' named entity 'hepatoma' named entity 'duplexes' named entity 'RNA interference' named entity 'siRNAs' named entity 'virus' named entity 'RNA sequencing' named entity 'Invitrogen' named entity 'RNA polymerase' named entity 'siRNAs' named entity 'genotype' named entity 'nucleotide' named entity 'HCV' named entity 'InvivoGen' named entity 'genome' named entity 'G418' named entity 'siRNA' named entity 'genome' named entity 'transfected' named entity 'HCV' named entity 'liver cirrhosis' named entity 'cDNA' named entity 'NTRs' named entity 'human immunodeficiency virus' named entity 'siRNAs' named entity 'PCR' named entity 'genetic diseases' named entity 'HCV' named entity 'HCV' named entity 'HCV' named entity 'siRNA' named entity 'negative control' named entity 'siRNA' named entity 'RNA' named entity 'RNA' named entity 'subgenomic' named entity 'antisense strand' named entity 'homologous' named entity 'base-pairing' named entity 'Huh7' named entity 'RNA replication' named entity 'hybridize' named entity 'genotype' named entity 'siRNAs' named entity 'Hepatoma' named entity 'hepatitis B virus'

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