Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. First described in 1942, FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. It was synthesized by Robert Seiwald and Joseph Burckhalter in 1958.
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| - Fluorescein isothiocyanate
- Isothiocyanate de fluorescéine
- Isotiocianato de fluoresceína
- Isotiocianato de fluoresceína
- Isotiocianato di fluoresceina
- إيزوثيوسيانات فلوريسين
- 异硫氰酸荧光素
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| - Isotiocianato de fluoresceína o fluoresceína-5-isotiocianato, también llamado FITC por sus siglas en inglés (Fluorescein IsoTioCyanate) es una molécula que pertenece al grupo de los colorantes de xanteno. Es un fluorocromo derivado de la fluoresceína. Los fluorocromos son moléculas capaces de emitir fluorescencia al ser excitadas a ciertas longitudes de onda. Cada fluorocromo presenta un espectro de absorción y de emisión característico. Entre los fluorocromos más usados en inmunohistoquímica figuran la fluoresceína, el isoticianato de fluoresceína, la rodamina B, el isotiocianato de rodamina B, el DANS y cloruro de dansilo. A pesar de que el isoticianato de fluoresceína se altera cuando se almacena durante periodos largos de tiempo, es más estable que la fluoresceína.
- La fluorescina isotiocianato (FITC) è il più comune derivato della fluorescina.Essa è un composto organico fluorescente dotata di un gruppo isotiocianato (-N=C=S) molto reattivo. Questo composto trova impiego in biologia in quanto il gruppo isotiocianato lega la fluorescina ai gruppi amminici e solfidrilici delle proteine permettendone la visualizzazione in . Il picco di assorbimento della FITC è all'incirca a 495 nm mentre quello di emissione in fluorescenza è sui 521 nm.
- Isotiocianato de fluoresceína (citado também com a sigla FITC, de seu nome em inglês fluorescein isothiocyanate) é um derivado da fluoresceína usado numa grande quantidade de aplicações como citometria de fluxo, (rítmo de filtração de glomerular), na marcação por conjugação de anticorpos, onde é o corante mais utilizado, etc. FITC é a molécula original de fluoresceína modificada com um grupo reativo isocianato (-N=C=S), substituindo um átomo de hidrogênio no anel inferior da estrutura. Este derivado é reativo porque atrai-se por grupos aminas e em proteínas.
- Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. First described in 1942, FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. It was synthesized by Robert Seiwald and Joseph Burckhalter in 1958.
- L'isothiocyanate de fluorescéine ou FITC est un dérivé de la fluorescéine, utilisé dans un large spectre d'applications comme la cytométrie en flux. Le FITC est une molécule de fluorescéine fonctionnalisée avec un groupe réactif isothiocyanate (-N=C=S), remplaçant un atome d'hydrogène sur le cycle le plus bas de la structure. Ce dérivé réagit avec les nucléophiles comme les groupes amines et sulfhydryles des protéines.
- إيزوثيوسيانات فلوريسين (بالإنجليزية: Fluorescein isothiocyanate) هو مركب مشتق من الفلورسين يستخدم في تطبيقات واسعة النطاق بما في ذلك قياس التدفق الخلوي. وصف لأول مرة في عام 1942، المركب عبارة عن جزيء فلوريسين موظف مع مجموعة إيزوثيوسيانات (-N = C = S)، لتحل محل ذرة الهيدروجين على الحلقة السفلية من الهيكل. يتوفر منه الكثير من الأيزومرات مثل فلورسين 5-أيزوثيوسيانات وفلوريسين 6-أيزوثيوسيانات. تم تصنيعه بواسطة روبرت سيوالد وجوزيف بوركهالتر في عام 1958.
- 异硫氰酸荧光素（英語：Fluorescein isothiocyanate，缩写FITC），是一种荧光素衍生物类荧光染料，广泛应用于流式細胞術和熒光顯微鏡，激发峰在495nm，发射峰在519nm。常用的FITC是两种异硫氰酸基官能团位置异构体5-FITC和6-FITC的化合物。
* FITC的激发光谱和发射光谱
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| - Isotiocianato de fluoresceína o fluoresceína-5-isotiocianato, también llamado FITC por sus siglas en inglés (Fluorescein IsoTioCyanate) es una molécula que pertenece al grupo de los colorantes de xanteno. Es un fluorocromo derivado de la fluoresceína. Los fluorocromos son moléculas capaces de emitir fluorescencia al ser excitadas a ciertas longitudes de onda. Cada fluorocromo presenta un espectro de absorción y de emisión característico. Entre los fluorocromos más usados en inmunohistoquímica figuran la fluoresceína, el isoticianato de fluoresceína, la rodamina B, el isotiocianato de rodamina B, el DANS y cloruro de dansilo. A pesar de que el isoticianato de fluoresceína se altera cuando se almacena durante periodos largos de tiempo, es más estable que la fluoresceína.
- Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. First described in 1942, FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. It was synthesized by Robert Seiwald and Joseph Burckhalter in 1958. A succinimidyl-ester functional group attached to the fluorescein core, creating "NHS-fluorescein", forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles. FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, giving it a green color. Like most fluorochromes, it is prone to photobleaching. Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed. In addition, some experiments make use of FITC's propensity for photobleaching in order to measure proteins' lateral mobility in membranes, through the technique of fluorescence recovery after photobleaching.
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