About: B1 protein: a novel cell penetrating protein for in vitro and in vivo delivery of HIV-1 multi-epitope DNA constructs

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About: B1 protein: a novel cell penetrating protein for in vitro and in vivo delivery of HIV-1 multi-epitope DNA constructs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	B1 protein: a novel cell penetrating protein for in vitro and in vivo delivery of HIV-1 multi-epitope DNA constructs
Creator	Azam, Kimia Elnaz, Bolhassani Hashemi, Agi
Source	PMC
abstract	OBJECTIVES: Enhancement of the potential ability of biomacromolecules to cross cell membranes is a critical step for development of effective therapeutic vaccine especially DNA vaccine against human immunodeficiency virus-1 (HIV-1) infection. The supercharged proteins were known as powerful weapons for delivery of different types of cargoes such as DNA and protein. Hence, we applied B1 protein with + 43 net charges obtained from a single frameshift in the gene encoding enhanced green fluorescent protein (eGFP) for delivery of two multi-epitope DNA constructs (nef-vpu-gp160-p24 and nef-vif-gp160-p24) in vitro and in vivo for the first time. For this purpose, B1 protein was generated in bacterial expression system under native conditions, and used to interact with both DNA constructs. RESULTS: Our data indicated that B1 protein (~ 27 kDa) was able to form a stable nanoparticle (~ 80–110 nm) with both DNA constructs at nitrogen: phosphate (N: P) ratio of 1:1. Moreover, the transfection efficiency of B1 protein for DNA delivery into HEK-293T cell line indicated that the cellular uptake of nef-vif-gp160-p24 DNA/ B1 and nef-vpu-gp160-p24 DNA/ B1 nanoparticles was about 32–35% with lower intensity as compared to TurboFect commercial reagent. On the other hand, immunization of BALB/c mice with different modalities demonstrated that B1 protein could enhance the levels of antibody, IFN-gamma and Granzyme B eliciting potent and strong Th1-directed cellular immunity. CONCLUSION: Generally, our findings showed the potency of B1 protein as a promising gene delivery system to improve an effective therapeutic vaccine against HIV-1 infection.
has issue date	2020-05-25(xsd:dateTime)
bibo:doi	10.1007/s10529-020-02918-w
has license	no-cc
sha1sum (hex)	13af98c04364a1cc9aaf5afc57a80b60c8b8d2f1
schema:url	https://doi.org/10.1007/s10529-020-02918-w
resource representing a document's title	B1 protein: a novel cell penetrating protein for in vitro and in vivo delivery of HIV-1 multi-epitope DNA constructs
has PubMed Central identifier	PMC7246087
schema:publication	Biotechnol Lett
resource representing a document's body	covid:13af98c04364a1cc9aaf5afc57a80b60c8b8d2f1#body_text
is schema:about of	named entity 'DNA' named entity 'p24' named entity 'Objectives' named entity 'protein' named entity 'cell' named entity 'DNA vaccine' named entity 'cargoes' named entity 'human immunodeficiency virus' named entity 'powerful' named entity 'net' named entity 'eGFP' named entity 'frameshift' named entity 'infection' named entity 'gene' named entity 'DNA vaccine' named entity 'protein' named entity 'noncovalently' named entity 'net charge' named entity 'cellular immune responses' named entity 'nucleic acid' named entity 'nanoparticles' named entity 'MTT' named entity 'vaccine' named entity 'nanoparticles' named entity 'immunoglobulin' named entity 'serum' named entity 'Granzyme B' named entity 'His-tag' named entity 'Student's t-test' named entity 'recombinant' named entity 'formazan' named entity 'IgG' named entity 'IgG1' named entity 'gene' named entity 'nanoparticles' named entity 'gene' named entity 'enzyme-linked immunosorbent assay' named entity 'gp160' named entity 'peptide' named entity 'dye' named entity 'positive charges' named entity 'protein' named entity '293T cells' named entity 'prokaryotic' named entity 'culture medium' named entity 'RPMI' named entity 'Abcam' named entity 'cationic' named entity 'mammalian cells' named entity 'nanoparticles' named entity 'multiepitope' named entity 'Pasteur Institute of Iran' named entity 'prime-boost' named entity 'administrated' named entity 'gene' named entity 'anionic' named entity 'gold nanoparticles' named entity 'kDa' named entity 'nanoparticle' named entity 'flow cytometry' named entity 'SEM' named entity 'Gentamicin' named entity 'peptides' named entity 'cell penetrating peptides' named entity 'transfection' named entity 'DNase' named entity 'net charge' named entity 'liposomes' named entity 'flow cytometry' named entity 'immunization' named entity 'recombinant' named entity 'molecule' named entity 'homologous proteins' named entity 'Iran' named entity 'supercharged' named entity 'immunization' named entity 'protein' named entity 'nanoparticles' named entity 'cytotoxicity' named entity 'gp160' named entity 'immunization'

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