About: Breeding of wastewater treatment yeasts that accumulate high concentrations of phosphorus

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About: Breeding of wastewater treatment yeasts that accumulate high concentrations of phosphorus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Breeding of wastewater treatment yeasts that accumulate high concentrations of phosphorus
Creator	Watanabe, Takashi Fujii, Tsutomu Iefuji, Haruyuki Iwashita, Kazuhiro Ozaki, Noriatsu
topic	covid:3cf80f975ff4395d7add1e82c9a8f4a36577b6dc#this
Source	PMC
abstract	Inorganic phosphate is an essential nutrient. In general, microorganisms take up phosphorus when the extracellular phosphorus concentration is low, but not when it is high. In Saccharomyces cerevisiae, the major phosphate transporters, such as Pho84p, and acid phosphatases (APases), such as Pho5p, are regulated in parallel by the phosphate signal transduction pathway (PHO pathway). We found that PHO mutants expressing PHO84 and PHO5, even under high-P conditions, could take up phosphorus at twice the rate of the wild-type strain. The regulatory pathway for phosphorus accumulation in two wastewater treatment yeasts, Hansenula fabianii J640 and Hansenula anomala J224-1, was found to be similar to that in S. cerevisiae. We screened for mutants of these yeasts that constitutively expressed APase. Such mutants formed blue colonies on high phosphorus concentration agar plates containing 5-bromo-4-chloro-3-indolylphosphate (X-phosphate). We found four mutants of H. fabianii J640 and one mutant of H. anomala J224-1 that accumulated from 2.2 to 3.5 times more phosphorus than the parent strains. The growth rates and abilities to remove dissolved total nitrogen and dissolved organic carbon of the mutants were similar to those of the parent strains. In addition, the mutants removed 95% of dissolved total phosphorus from shochu wastewater, while the parent strain removed only 50%.
has issue date	2008-08-01(xsd:dateTime)
bibo:doi	10.1007/s00253-008-1529-8
bibo:pmid	18604532
has license	no-cc
sha1sum (hex)	3cf80f975ff4395d7add1e82c9a8f4a36577b6dc
schema:url	https://doi.org/10.1007/s00253-008-1529-8
resource representing a document's title	Breeding of wastewater treatment yeasts that accumulate high concentrations of phosphorus
has PubMed Central identifier	PMC2491421
has PubMed identifier	18604532
schema:publication	Appl Microbiol Biotechnol
resource representing a document's body	covid:3cf80f975ff4395d7add1e82c9a8f4a36577b6dc#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/3cf80f975ff4395d7add1e82c9a8f4a36577b6dc
is schema:about of	named entity 'concentration' named entity 'essential nutrient' named entity 'extracellular' named entity 'parent' named entity 'wastewater treatment' named entity 'TOTAL PHOSPHORUS' named entity 'SIMILAR' named entity 'SACCHAROMYCES CEREVISIAE' named entity 'BLUE' named entity 'BROMO' named entity 'TWICE' named entity 'ESSENTIAL NUTRIENT' named entity 'STRAINS' named entity 'DISSOLVED ORGANIC CARBON' named entity 'TIMES' named entity 'THESE' named entity 'MUTANT' named entity 'remove' named entity 'dissolved' named entity 'phosphorus' named entity 'agar' named entity 'mutants' named entity 'phosphatases' named entity 'Saccharomyces cerevisiae' named entity 'Hansenula anomala' named entity 'wastewater treatment' named entity 'Hansenula' named entity 'phosphorus' named entity 'wild-type' named entity 'regulatory pathway' named entity 'acid phosphatase' named entity 'wastewater' named entity 'trace elements' named entity 'yeast cells' named entity 'phosphate' named entity 'downregulated' named entity 'vacuoles' named entity 'eluted' named entity 'activated sludge' named entity 'wastewater' named entity 'wastewater' named entity 'phosphorus' named entity 'dextrose' named entity 'PHO' named entity 'shochu' named entity 'phosphorus' named entity 'sweet potato' named entity 'hydrolysis' named entity 'distilleries' named entity 'phosphate transporter' named entity 'barley' named entity 'phosphate' named entity 'centrifuged' named entity 'inoculate' named entity 'wastewater' named entity 'phosphorus' named entity 'inorganic phosphate' named entity 'pH 4' named entity 'phosphate' named entity 'hydrolyze' named entity 'wastewater treatment' named entity 'barley' named entity 'UV light' named entity 'Acid phosphatase' named entity 'flocculating' named entity 'acid phosphatase' named entity 'extracellular' named entity 'phosphorus' named entity 'organic compounds' named entity 'naphthyl' named entity 'inorganic phosphate' named entity 'S. cerevisiae' named entity 'phosphorus' named entity 'phosphate' named entity 'yeast strains'

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