About: LRRK2-phosphorylated Rab10 sequesters Myosin Va with RILPL2 during ciliogenesis blockade

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About: LRRK2-phosphorylated Rab10 sequesters Myosin Va with RILPL2 during ciliogenesis blockade Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	LRRK2-phosphorylated Rab10 sequesters Myosin Va with RILPL2 during ciliogenesis blockade
Creator	Pfeffer, Suzanne Yanatori, Izumi Dhekne, Herschel Federico Diez, Sobu, Yuriko Vides, Edmundo
Source	BioRxiv
abstract	Activating mutations in LRRK2 kinase cause Parkinson’s disease. Pathogenic LRRK2 phosphorylates a subset of Rab GTPases and blocks ciliogenesis. Thus, defining novel phospho-Rab interacting partners is critical to our understanding of the molecular basis of LRRK2 pathogenesis. RILPL2 binds with strong preference to LRRK2-phosphorylated Rab8A and Rab10. RILPL2 is a binding partner of the motor protein and Rab effector, Myosin Va. We show here that the globular tail domain of Myosin Va also contains a high affinity binding site for LRRK2-phosphorylated Rab10, and certain tissue-specific Myosin Va isoforms strongly prefer to bind phosphorylated Rab10. In the presence of pathogenic LRRK2, RILPL2 relocalizes to the peri-centriolar region in a phosphoRab10- and Myosin Va-dependent manner. In the absence of phosphoRab10, expression of RILPL2 or depletion of Myosin Va increase centriolar RILPL2 levels, and either condition is sufficient to block ciliogenesis in RPE cells. These experiments show that LRRK2 generated phosphoRab10 dramatically redistributes Myosin Va-RILPL2 complexes to the mother centriole, which may sequester Myosin Va and RILPL2 in a manner that blocks their normal roles in ciliogenesis.
has issue date	2020-04-28(xsd:dateTime)
bibo:doi	10.1101/2020.04.28.065664
has license	biorxiv
sha1sum (hex)	79ff43715f7626d980f00f7cb1dc96bbdef1184f
schema:url	https://doi.org/10.1101/2020.04.28.065664
resource representing a document's title	LRRK2-phosphorylated Rab10 sequesters Myosin Va with RILPL2 during ciliogenesis blockade
schema:publication	bioRxiv
resource representing a document's body	covid:79ff43715f7626d980f00f7cb1dc96bbdef1184f#body_text
is schema:about of	named entity 'Myosin' named entity 'sequester' named entity 'condition' named entity 'mother' named entity 'phosphorylated' named entity 'subset' named entity 'block' named entity 'Myosin' named entity 'phosphorylated' named entity 'manner' named entity 'pathogenic' named entity 'presence' named entity 'Rab' named entity 'bind' named entity 'blocks' named entity 'binds' named entity 'mutations' named entity 'centriole' named entity 'Rab' named entity 'Rab GTPases' named entity 'Myosin' named entity 'pathogenesis' named entity 'phosphorylated' named entity 'Rab10' named entity 'Myosin' named entity 'binding site' named entity 'LRRK2' named entity 'tail domain' named entity 'LRRK2' named entity 'phosphorylated' named entity 'lentivirus' named entity 'kinase' named entity 'phosphorylation' named entity 'HEK293T cells' named entity 'rat' named entity 'exogenous' named entity 'protein' named entity 'Rab10' named entity 'sodium orthovanadate' named entity 'charge coupled device' named entity 'hTERT' named entity 'Rab10' named entity 'mother centriole' named entity 'serum' named entity 'phosphorylation' named entity 'Fiji' named entity 'exons' named entity 'Rab10' named entity 'Cell culture' named entity 'ciliogenesis' named entity 'Rab10' named entity 'non-linear regression' named entity 'phenotype' named entity 'physiological functions' named entity 'Exon' named entity 'protein' named entity 'GTP' named entity 'serum' named entity 'A549' named entity 'endocrine' named entity 'primary cilia' named entity 'Rab11A' named entity 'Trizol' named entity 'phosphorylated' named entity 'LRRK2' named entity 'immunoblotting' named entity 'Rab8' named entity 'mother centriole' named entity 'gene knockdown' named entity 'endogenous' named entity 'staining' named entity 'CEP164' named entity 'primary cilia' named entity 'Gibson assembly' named entity 'imidazole' named entity 'ciliogenesis' named entity 'Rab10' named entity 'Exon' named entity 'oligodendrocyte precursor cells'

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